PMID: 11913497Mar 27, 2002Paper

Quantitative analysis of hepatitis B virus DNA by real-lime amplification

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
I ZanellaE Cariani

Abstract

Diagnostic assays allowing the quantification of hepatitis B virus (HBV) DNA over a wide range of concentrations are important for monitoring patients during antiviral therapy. The aim of this study was to develop a new real-time method for HBV DNA quantification. Primers and probe were selected in a highly conserved region of the HBV S gene, and a plasmid containing the pre-S/S region was used as a standard. Linear quantification of the standard was obtained between 10 and 10(9) copies/reaction, with high correlation between ayw and adw genomes (P<0.001). HBV DNA was detected in serial dilutions of a high-titer serum sample with linear results until 2.4 x 10(3) copies/ml. One hundred eight serum samples positive for hepatitis B surface antigen were tested in both the real-time assay and the Digene Hybrid Capture assay (Digene, USA). HBV DNA could be detected by both assays in 70 samples, with significant correlation of results (P<0.001). Results for 38 samples were below the sensitivity limit of the Digene assay, but they could be quantified by the real time polymerase chain reaction assay. These results show that real-time polymerase chain reaction allows sensitive, rapid and linear quantification of HBV DNA in serum.

Citations

Apr 10, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·J WeissJ Siegel
May 6, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sani Hussein AliyuMartin David Curran
Jul 24, 2002·Biochemical and Biophysical Research Communications·Ming-Liang HeHsiang-fu Kung
May 3, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Stephan SchaeferWolfram H Gerlich
May 1, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Perumal VivekanandanMichael Torbenson
Mar 24, 2009·The Journal of Infectious Diseases·Perumal VivekanandanMichael Torbenson
Sep 22, 2007·Acta Pharmacologica Sinica·Zong-Yan ChenZhan Li
Jan 19, 2006·Clinical Microbiology Reviews·M J EspyT F Smith
Sep 7, 2006·Journal of Clinical Microbiology·Tania M WelzelBetty A Ortiz-Conde
Dec 22, 2006·Journal of Clinical Microbiology·Ying LiuAnna S F Lok
Jun 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tokiko WatanabePaul Ahlquist
Jan 11, 2008·Journal of Viral Hepatitis·P VivekanandanM Torbenson
Aug 23, 2005·Human Pathology·Morishige TakeshitaFujio Matsubara
Sep 18, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Danielle Alves Gomes ZauliAlessandro Clayton de Souza Ferreira
Dec 25, 2007·The Korean journal of laboratory medicine·Jeong HeoEeu Yup Lee
Dec 25, 2007·The Korean journal of laboratory medicine·Sang Hyun HwangHeung Bum Oh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.