PMID: 16531769Mar 15, 2006Paper

Quantitative analysis of herpesvirus load in the lymph nodes of patients with histiocytic necrotizing lymphadenitis using a real-time PCR assay

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
Nagako MaedaNaoyoshi Mori

Abstract

The cause of histiocytic necrotizing lymphadenitis (HNL) has been ascribed to viral infection, but its pathogenesis still remains unknown. Real-time PCR assays are useful not only for their sensitivity of detection but also for the quantitation of viral DNA with a wide linear range. We accordingly used this technique to estimate for each patient the viral load of the following members of the herpesvirus family: Epstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpesvirus (HHV) types 6, 7, and 8. Samples of patients diagnosed as reactive lymphadenopathy (RL) were included for control. Thirty percent (6/20 cases) and 63% (12/19 cases) of the HNL and RL patients were positive for EBV, and the mean of the detectable EBV viral load of the HNL and that of the RL patients were 463 and 355 (copies/mug DNA), respectively. By in situ hybridization, EBV-encoded RNA could be detected in the lymph tissue samples with more than 14.3 copies/mug of EBV DNA. No significant difference was detected between the number of HNL patients with HHV6 DNA (3/20, 15%) or HHV7 DNA (2/20, 10%) and RL controls. CMV and HHV8 were not detected in the DNA from any patient. In this study, we were unable to definitively identify the causative herpesvirus...Continue Reading

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Citations

Nov 20, 2012·Rheumatology International·Jorge LabradorRamon García-Sanz
Nov 12, 2009·Indian Journal of Pediatrics·Chao Chun ZouLi Liang
Dec 18, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Yoriko YamashitaTatsuya Tsurumi
Sep 10, 2014·International Journal of Pediatric Otorhinolaryngology·Yosep Chong, Chang Suk Kang
Dec 4, 2012·La Revue de médecine interne·C MéniS Mouly
Jan 7, 2014·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·María Dolores FellnerMaría Alejandra Picconi
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Nov 26, 2008·Revista médica de Chile·José Manuel López M
May 26, 2012·Proceedings. Biological Sciences·Matthew R WalshDavid M Post
Oct 23, 2013·Cornea·Tomoyuki Inoue, Yuichi Ohashi

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