Quantitative analysis of histamine in biological samples by gas chromatography--mass spectrometry

Journal of Chromatography
H MitaT Shida

Abstract

A mass fragmentographic method for the quantitative analysis of histamine in the supernatant from antigen-challenged leukocytes, whole blood, and urine is described. Histamine labeled with two 15N atoms was synthesized and added to the sample as an internal standard. N alpha-Heptafluorobutyryl-N tau-ethoxycarbonylhistamine was prepared for mass fragmentographic analysis and the molecular ions at m/z 379 and 381 were used for monitoring histamine and 15N2-labeled histamine, respectively. The quantitation limit of histamine was 2 ng by this method. The experimental error of the method was less than 7% at the level of 5 ng in the supernatant from antigen-challenged leukocytes. The value obtained by this method correlated well with that from radioisotopic enzymatic assay (r=0.990).

Citations

Oct 1, 1987·Agents and Actions·J P GreenP Blandina
Sep 14, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Anna Sophie ZimmermannDetlef Neumann
Nov 11, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·L GuillouxG Ville
Jan 1, 1984·Journal of Pharmaceutical and Biomedical Analysis·A Hulshoff, H Lingeman
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shigeyuki Oguri, Yukari Yoneya
May 29, 2008·Biomedical Chromatography : BMC·Toshimasa Toyo'oka
Mar 1, 1991·The Journal of Investigative Dermatology·L J Roberts, J A Oates
Jan 1, 1983·Clinical Allergy·P W IndC T Dollery
Jan 1, 1986·Critical Reviews in Toxicology·S L Taylor
Apr 3, 2020·Analytical and Bioanalytical Chemistry·A R PereiraC M P Gomes Amorim
Aug 9, 2002·Journal of Agricultural and Food Chemistry·Francis R AntoineMaurice R Marshall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.