Quantitative Analysis of Immunosuppressive Drugs Using Tungsten Disulfide Nanosheet-Assisted Laser Desorption Ionization Mass Spectrometry.

ACS Nano
Sunho JohTae Geol Lee

Abstract

For organ transplantation patients, the therapeutic drug monitoring (TDM) of immunosuppressive drugs is essential to prevent the toxicity or rejection of the organ. Currently, TDM is done by immunoassays or liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods; however, these methods lack specificity or are expensive, require high levels of skill, and offer limited sample throughput. Although matrix-assisted (MA) laser desorption ionization (LDI) mass spectrometry (MS) can provide enhanced throughput and cost-effectiveness, its application in TDM is limited due to the limitations of the matrixes such as a lack of sensitivity and reproducibility. Here, we present an alternative quantification method for the TDM of the immunosuppressive drugs in the blood of organ transplant patients by utilizing laser desorption ionization mass spectrometry (LDI-MS) based on a tungsten disulfide nanosheet, which is well-known for its excellent physicochemical properties such as a strong UV absorbance and high electron mobility. By adopting a microliquid inkjet printing system, a high-throughput analysis of the blood samples with enhanced sensitivity and reproducibility was achieved. Furthermore, up to 80 cases of patient samples were...Continue Reading

References

Oct 28, 1989·Lancet·T E StarzlA Jain
May 30, 2002·Analytical Chemistry·Zhong GuoJianyi Ni
Jan 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Titte R SrinivasBruce Kaplan
Sep 13, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sara BaldelliDario Cattaneo
Feb 27, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·GholamAli KhoschsorurGabriele Halwachs-Baumann
May 9, 2008·Acta Paediatrica·Anthony K Akobeng
Dec 25, 2008·Briefings in Functional Genomics & Proteomics·Mark W DuncanStephen W Hunsucker
Aug 24, 2010·Analytical and Bioanalytical Chemistry·K P Law, James R Larkin
Apr 3, 2012·Chemical Communications : Chem Comm·Brian CreranVincent M Rotello
Jun 19, 2012·Journal of Environmental Monitoring : JEM·Christopher SzakalR David Holbrook
Feb 23, 2013·Analytica Chimica Acta·Jie-Bi HuPawel L Urban
Mar 12, 2013·Scientific Reports·Kun QianChengzhong Yu
Apr 16, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wlodzimierz TszyrsznicMichal Dadlez
May 22, 2013·American Journal of Clinical Pathology·Darla R LowerAdam Rosendorff
Sep 3, 2013·Journal of Pharmaceutical and Biomedical Analysis·Filippo AucellaElvira Grandone
Mar 13, 2014·Analytical Chemistry·Christopher SzakalR David Holbrook
Jan 23, 2015·Journal of the American Chemical Society·Stanley S ChouVinayak P Dravid
Jul 24, 2015·Journal of the American Society for Mass Spectrometry·Alyssa L M MarsicoRichard W Vachet
Aug 12, 2015·The Journal of Physical Chemistry Letters·Amit GuptaSukumaran Vasudevan
Feb 15, 2016·Journal of Pharmaceutical and Biomedical Analysis·Adriana Mika, Piotr Stepnowski
Apr 4, 2017·Nanomaterials·Rosaria Anna PiccaFrancesco Palmisano
Aug 30, 2017·Nanoscale·Maciej R MolasMarek Potemski
Sep 27, 2018·Journal of Food and Drug Analysis·Han-Wei ChuChih-Ching Huang
Jan 31, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Belén Rodríguez-SánchezGilbert Greub
Feb 17, 2019·Nanomaterials·Pawel Pomastowski, Boguslaw Buszewski
Jul 10, 2019·Nano Letters·Jimmy C KotsakidisMichael S Fuhrer

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Citations

Aug 10, 2021·Journal of the American Society for Mass Spectrometry·Yaju ZhaoDanke Xu

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