Quantitative analysis of inflammatory and immune responses in dogs with gastritis and their relationship to Helicobacter spp. infection

Journal of Veterinary Internal Medicine
Bo WiinbergKenneth W Simpson

Abstract

The present study sought to quantitatively examine mucosal inflammatory and immune responses in dogs with gastritis and the relationship of these responses to infection with Helicobacter. Gastric biopsies from 30 dogs were evaluated for B- and T-lymphocytes, neutrophils, eosinophils, macrophages, and mast cells. Mucosal atrophy, fibrosis, cellularity, and severity of gastritis were graded qualitatively. Messenger-RNA (mRNA) for actin, interleukin-1beta (IL-1beta), IL-4, IL-8, and IL-10, transforming growth factor beta (TGF-beta), and interferon gamma (IFN-gamma) was quantified by polymerase chain reaction (PCR). The presence of Helicobacter spp. was determined by urease activity, histology, PCR, and enzyme-linked immunosorbent assay. mRNA for IL-1beta, IL-8, IL-10, TGF-beta, and IFN-gamma was detected in most dogs. IL-4 mRNA was detected in only 1 dog. Correlations were observed for IL-1beta versus IL-8 and IL-10; IL-8 versus IL-10, IFN-gamma, and TGF-beta; and IL-10 versus IFN-y. Mucosal pathology was related to cytokine mRNA expression (neutrophils to IL-8 and IFN-gamma, macrophages and lymphocytes to IFN-gamma, and fibrosis to IL-1beta). Gastritis was categorized as lymphoplasmacytic in all dogs, and its histologic severity ...Continue Reading

Citations

Oct 26, 2011·American Journal of Veterinary Research·Melanie CravenKenneth W Simpson
Apr 16, 2009·Clinical Microbiology Reviews·Freddy HaesebrouckRichard Ducatelle
Aug 26, 2009·Helicobacter·Camilla RecordatiEugenio Scanziani
Jun 6, 2007·The Journal of Small Animal Practice·C TyszkoS L Swist
Apr 21, 2007·Veterinary Ophthalmology·Carrie B BreauxBruce H Grahn
Feb 9, 2010·The Journal of Small Animal Practice·C ElwoodK Savary-Bataille
Oct 24, 2013·Veterinary Immunology and Immunopathology·Yu TamuraMitsuyoshi Takiguchi
Jul 10, 2012·Veterinary Parasitology·E DvirJ P Schoeman
Jul 9, 2016·The Veterinary Journal·Albert E JergensKarin Allenspach
Oct 13, 2006·Veterinary Microbiology·Camilla RecordatiEugenio Scanziani
Mar 22, 2013·Acta Veterinaria Scandinavica·Tonje Seim-WikseEllen Skancke
Apr 22, 2015·BMC Veterinary Research·Anna TauroClare Rusbridge
Apr 15, 2010·Journal of Veterinary Internal Medicine·R J WashabauUNKNOWN WSAVA International Gastrointestinal Standardization Group
Feb 13, 2021·Veterinary Medicine International·Daniel D Guerra SegundoSaulo T L Pinto Filho

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