Abstract
A new method is presented for the quantitative analysis of intercellular adhesive specificity. In this assay, two cell types are mixed, one unlabeled and the other labeled with the fluorescent dye, fluorescamine [4-phenylspiro(feran-2[3H],1'-phthalan)-3,3'-dione]. The resulting aggregates are analyzed by fluorescence microscopy to determine the number of labeled and unlabeled cells per aggregate. Random (nonspecific) aggregation was characterized by a binomial distribution, and adhesive specificity was accordingly quantified by the deviation (as determined by a chi-square test) from the calculated binomial distribution. The labeling procedure was simple and rapid, and experiments with 18 different cell types showed that it did not affect cell viability, morphology, rate and extent of adhesion, plating efficiency, and the capability of myogenic cells to undergo terminal differentiation. Most important, assays with morphologically identifiable cell pairs indicated that the fluorescent label neither induced apparent nor destroyed existing adhesive specificity. The most pronounced adhesive specificities were observed with freshly explanted cells from adult tissues and also with mixtures of simian virus 40-transformed and nontransfo...Continue Reading
References
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J M Pouysségur, I Pastan
Aug 1, 1977·Endocrinology·P M ConnK J Catt
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J PouysségurI Pastan
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B ObrinkS Roseman
Jan 1, 1979·Annual Review of Biochemistry·W Frazier, L Glaser
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K WeberM Osborn
May 1, 1979·Developmental Biology·M TakeichiT S Okada
Jul 1, 1979·Journal of Cellular Physiology·M A CifoneR M Baker
Apr 5, 1978·Journal of Molecular Biology·S Bozzaro, G Gerisch
Jun 1, 1978·Archives of Biochemistry and Biophysics·E J Neufeld, I Pastan
Sep 1, 1978·The Journal of Cell Biology·W R Springer, S H Barondes
Dec 14, 1976·Biochimica Et Biophysica Acta·R B MarchaseS Roth
Sep 9, 1977·Biochemical and Biophysical Research Communications·G H Rothblat, F D DeMartinis
Feb 23, 1976·Biochemical and Biophysical Research Communications·S P HawkesM J Bissell
Sep 1, 1976·The Journal of Clinical Investigation·R L VanD A Roncari
Oct 1, 1976·The Journal of Cell Biology·S R Grady, E J McGuire
Oct 1, 1975·Cell·P StanleyL Siminovitch
Feb 1, 1976·The Journal of Cell Biology·H GershmanL Culp
Jan 1, 1973·The Journal of Membrane Biology·M S SteinbergR E Granger
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B T WaltherS Roseman
Aug 1, 1973·Developmental Biology·J K Dorsey, S Roth
Nov 1, 1971·The Journal of Cell Biology·S RothS Roseman
Jul 30, 1974·Biochemistry·P J SimsJ F Hoffman
Nov 24, 1972·Science·S UdenfriendM Weigele
Mar 10, 1971·Nature: New Biology·C P StannersH Green
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·G M EdelmanC F Millette
Oct 1, 1970·Chemistry and Physics of Lipids·S Roseman
Jul 1, 1959·Virology·R DULBECCO, G FREEMAN
Citations
Dec 1, 1985·Developmental Biology·D Gibralter, D C Turner
Jul 1, 1985·Journal of the Neurological Sciences·J A Pizzey, G E Jones
Jan 1, 1983·Pharmacology & Therapeutics·S Massa, H B Bosmann
Dec 1, 1988·The Journal of Cell Biology·J A PizzeyF S Walsh
Dec 1, 1989·The Journal of Cell Biology·J A PizzeyF S Walsh
Jul 1, 1993·The Journal of Cell Biology·M T Filbin, G I Tennekoon
Jan 1, 1994·The Journal of Cell Biology·H M DeLisserS M Albelda
May 1, 1992·The Journal of Experimental Medicine·W A MullerS M Albelda
Sep 23, 1997·The Journal of Cell Biology·J FamigliettiS M Albelda
Feb 14, 2016·Evaluation and Program Planning·Sumera Jabeen
May 7, 2009·Journal of Nursing Management·Hannah Cooke
Mar 1, 1984·Cytometry·D M Segal, D A Stephany
Dec 20, 2005·International Journal of Nursing Studies·Hannah Cooke
Aug 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·F BreviarioE Dejana
Apr 26, 1990·Nature·M T FilbinG I Tennekoon
Oct 6, 1995·The Journal of Biological Chemistry·H C YanH M DeLisser
Apr 1, 2000·The Journal of Biological Chemistry·K Satoh-HorikawaY Takai
Aug 2, 1996·The Journal of Biological Chemistry·J SunH M DeLisser
Jan 5, 2000·The Journal of Biological Chemistry·M MiyaharaY Takai
Feb 1, 1995·Molecular and Cellular Biology·P BellostaC Basilico