Quantitative analysis of KLF4 and SOX2 expression in oral carcinomas reveals independent association with oral tongue subsite location and histological grade.

Cancer Biomarkers : Section a of Disease Markers
María Luisa PaparellaAna Rosa Raimondi

Abstract

Stemness factors associated with tumorigenesis in different types of cancers have not been specifically studied in oral tongue SCC (OTSSC). Here, we aimed to quantify expression levels and distribution of KLF4 and SOX2, two relevant stemness factors, in oral SCC including OTSCC samples from different subsites. We determined KLF4 and SOX2 expression levels by immunostaining 35 biopsies of OSCC. Stained wholeslide images were digitized and subjected to automatic cell detection and unbiased quantification using Qupath software. We found statistically significant reduction in KLF4 positive cells density (p= 0.024), and fraction (p= 0.022) in OTSCC from tongue borders compared with other tongue subsites. Instead, quantitative SOX2 analysis did not show differences in expression levels between OTSCC from the borders versus OTSCC developed in others subsites. Notably SOX2 expression was revealed increased in moderately and poorly differentiated OSCC compared with well differentiated ones (positive cells density p= 0.025, fraction p= 0.006). No significant correlation between KLF4 and SOX2 expression was observed, neither in OSCC nor in OTSCC. KLF4 and SOX2 exhibit opposite expression profiles regarding subsite localization and differe...Continue Reading

References

Jun 22, 2002·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Masumi OkamotoNoboru Konishi
Sep 4, 2003·Genome Biology·Dov GreenbaumMark Gerstein
Dec 24, 2005·Nature Reviews. Cancer·Benjamin D Rowland, Daniel S Peeper
May 4, 2006·The Journal of Clinical Investigation·Julia A Segre
Jun 13, 2006·Journal of Cancer Research and Clinical Oncology·K M SathyanS Kannan
Sep 5, 2007·Cancer Metastasis Reviews·Daisuke Sano, Jeffrey N Myers
Sep 23, 2008·Oral Oncology·Saman Warnakulasuriya
Dec 31, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Avraham ZiniHarold D Sgan-Cohen
Jul 20, 2010·Oral Oncology·Ibrahim O BelloTuula Salo
Jan 10, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geoffrey T HartStephen C Jameson
Apr 27, 2013·Cancer Discovery·Curtis R PickeringMitchell J Frederick
Sep 26, 2013·Nature Reviews. Cancer·Marie-Pier TetreaultJonathan P Katz
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Jan 22, 2015·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·María L PaparellaAna R Raimondi
May 5, 2016·Scientific Reports·Idit KostiMarina Sirota
Oct 11, 2016·The New England Journal of Medicine·Robert L FerrisMaura L Gillison
Feb 27, 2017·Gene·Amr M Ghaleb, Vincent W Yang
Jun 28, 2017·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Aisha A HusseinTymour Forouzanfar
Nov 9, 2017·Nature Reviews. Clinical Oncology·Ibiayi Dagogo-Jack, Alice T Shaw
Dec 6, 2017·Scientific Reports·Peter BankheadPeter W Hamilton
May 18, 2018·Journal of Dental Research·J E Nör, J S Gutkind
Jun 18, 2019·Journal of Cellular Physiology·Si Si Yu, Nicola Cirillo
Aug 15, 2019·Seminars in Cancer Biology·Daniel NovakJochen Utikal
Oct 11, 2019·Precision Clinical Medicine·Demeng Chen, Cun-Yu Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.