Quantitative analysis of lactase-phlorizin hydrolase expression in the absorptive enterocytes of newborn rat small intestine

Journal of Cellular Physiology
G EstradaM D López-Tejero

Abstract

At birth, the mammalian small intestine displays regional differences in morphology as well as complex proximal-to-distal (horizontal) patterns of protein distribution. Lactase-phlorizin hydrolase (LPH), an enterocyte-specific disaccharidase crucial for the digestion of lactose in milk, reveals a characteristic horizontal pattern of expression at birth. However, it is not certain whether this topographic pattern is due to variations in epithelial structure along the length of the small intestine or to regional differences in the transcription of the LPH gene. In order to understand the mechanisms that regulate the regionalization of LPH at birth, we characterized the epithelial structure along the horizontal axis using stereologic techniques and correlated these data with the patterns of lactase activity and LPH mRNA abundance in the small intestine of unsuckled, newborn rats. Epithelial volume and microvillar surface area per unit of intestinal length decreased three-to fourfold from duodenum to distal ileum. In contrast, lactase activity and LPH mRNA abundance were highest in proximal jejunum and lowest in the most proximal and distal ends of the small intestine. Mean lactase activity per cell in proximal duodenum, proximal j...Continue Reading

References

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Citations

Aug 21, 2004·Journal of Pediatric Gastroenterology and Nutrition·Menno VerhaveRobert K Montgomery
Dec 1, 1996·Alcoholism, Clinical and Experimental Research·G EstradaM D López-Tejero
Sep 3, 1998·Alcoholism, Clinical and Experimental Research·G EstradaM D López-Tejero
Dec 8, 1998·Pathology, Research and Practice·S E KongJ Hall

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