PMID: 6165996Jan 1, 1981Paper

Quantitative analysis of melanoma-associated antigen p97 in normal and neoplastic tissues

Proceedings of the National Academy of Sciences of the United States of America
J P BrownK E Hellström

Abstract

We have used two highly sensitive assays to quantitate p97, a protein associated with human melanoma, in cultured cells and normal adult, fetal, and neoplastic tissues. To measure p97 at the surface of intact cells, radiolabeled Fab fragments of a monoclonal antibody specific for p97 were used in a binding assay. To measure p97 in detergent-solubilized membrane preparations, we used a novel double-determinant immunoassay that uses two monoclonal antibodies to two distinct antigenic determinants of p97. These assays revealed that although p97 is present in small amounts in normal adult tissues, it is present in much larger amounts in most melanomas, in some other tumors (both benign and malignant), and in certain fetal tissues. We conclude that monoclonal antibodies to p97 may prove to be of value for the diagnosis and therapy of melanoma.

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Y YehK E Hellström
Jan 1, 1966·International Archives of Allergy and Applied Immunology·P J McConahey, F J Dixon
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D M ThomsonP Gold
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R G WoodburyK E Hellström

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Citations

Jul 5, 1997·Biotechnology and Bioengineering·M L KennardW A Jefferies
Nov 1, 1991·Journal of Cellular Biochemistry·A RayChaudhury, P A D'Amore
Nov 1, 1995·European Journal of Nuclear Medicine·S E Halpern, R Bartholomew
Jan 1, 1985·The Journal of Membrane Biology·W S May, P Cuatrecasas
Nov 5, 1991·Journal of Immunological Methods·R AbrahamK E Hellström
Jun 8, 1987·Life Sciences·P BittermanD M Gersten
Nov 1, 1988·Molecular Immunology·D M SegalJ R Wunderlich
Feb 21, 1995·Biochimica Et Biophysica Acta·A RozéN Duchange
Feb 19, 1991·Biochimica Et Biophysica Acta·D R Richardson, E Baker
Feb 1, 1983·European Journal of Cancer & Clinical Oncology·L MedranoM Prunieras
Jun 7, 2005·Experimental Cell Research·Jonathan Michaud-LevesqueRichard Béliveau
Mar 30, 2002·Critical Reviews in Oncology/hematology·Juliana C Kwok, Des R Richardson
Nov 1, 1996·Nature Medicine·M L KennardW A Jefferies
Nov 8, 2002·Journal of Neurochemistry·Michel DemeuleRichard Béliveau
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G T NepomK E Hellström
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T M RoseJ P Brown
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C D EstinK E Hellström
Feb 1, 1989·The Journal of Experimental Medicine·K S FurukawaK O Lloyd
Apr 1, 1985·Annals of Surgery·H F Seigler
Aug 7, 1999·APMIS. Supplementum·H J Ditzel
Nov 20, 2008·Pigment Cell & Melanoma Research·Yannève RollandRichard Béliveau
Dec 1, 1983·The Journal of Clinical Investigation·S M LarsonK E Hellström
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·S ParkkilaW S Sly
Jan 1, 1992·Journal of Cancer Research and Clinical Oncology·S H de BieF J Cleton

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