Quantitative analysis of mitochondrial membrane potential heterogeneity in unsynchronized and synchronized cancer cells.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Amandine RoviniEduardo N Maldonado

Abstract

Mitochondrial membrane potential (ΔΨm) is a global indicator of mitochondrial function. Previous reports on heterogeneity of ΔΨm were qualitative or semiquantitative. Here, we quantified intercellular differences in ΔΨm in unsynchronized human cancer cells, cells synchronized in G1, S, and G2, and human fibroblasts. We assessed ΔΨm using a two-pronged microscopy approach to measure relative fluorescence of tetramethylrhodamine methyl ester (TMRM) and absolute values of ΔΨm. We showed that ΔΨm is more heterogeneous in cancer cells compared to fibroblasts, and it is maintained throughout the cell cycle. The effect of chemical inhibition of the respiratory chain and ATP synthesis differed between basal, low and high ΔΨm cells. Overall, our results showed that intercellular heterogeneity of ΔΨm is mainly modulated by intramitochondrial factors, it is independent of the ΔΨm indicator and it is not correlated with intercellular heterogeneity of plasma membrane potential or the phases of the cell cycle.

References

Jan 1, 1976·Zeitschrift Für Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology·S Weinhouse
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S T SmileyL B Chen
Jan 1, 1995·Methods in Enzymology·M ReersL B Chen
Jan 6, 1999·Biophysical Journal·R C Scaduto, L W Grotyohann
Apr 3, 2002·The EMBO Journal·Tony J CollinsMartin D Bootman
Aug 10, 1956·Science·S WEINHOUSE
Nov 5, 2004·Molecular Cell·Hugh CamBrian David Dynlacht
Jan 6, 2007·PloS One·Marta Martínez-DiezJosé M Cuezva
Apr 3, 2007·Biochemical and Biophysical Research Communications·Seungmin LeeHyeseong Cho
May 23, 2007·Journal of Biomolecular Screening·Michael P MaherHong Ao
Jan 1, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Felix DistelmaierPeter H G M Willems
Jun 28, 2008·Cell Cycle·Stefan M SchiekeToren Finkel
Oct 22, 2008·Methods : a Companion to Methods in Enzymology·Werner J H KoopmanPeter H G M Willems
May 23, 2009·Science·Matthew G Vander HeidenCraig B Thompson
May 27, 2009·International Journal of Molecular Sciences·Andrey V Kuznetsov, Raimund Margreiter
Jul 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kasturi MitraJennifer Lippincott-Schwartz
Jul 29, 2009·Chembiochem : a European Journal of Chemical Biology·Lema F YousifShana O Kelley
Mar 7, 1927·The Journal of General Physiology·O WarburgE Negelein
Dec 17, 2010·Cancer Research·Eduardo N MaldonadoJohn J Lemasters
Dec 24, 2010·PLoS Biology·Ricardo Pires das NevesFrancisco J Iborra
Jan 18, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zhuohui GanMarilyn P Merker
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Jul 6, 2011·The Biochemical Journal·Martin D Brand, David G Nicholls
Mar 9, 2013·The Journal of Biological Chemistry·Eduardo N MaldonadoJohn J Lemasters
Oct 3, 2015·Cell Cycle·Joanna KaluckaPeter Carmeliet
Dec 18, 2015·Cell Metabolism·Madhusudhanan SukumarNicholas P Restifo
Jan 29, 2016·Stem Cells Translational Medicine·Ayako KumagaiMiho K Furue
Dec 10, 2016·Experimental & Molecular Medicine·Ji Hoon JeonHyonchol Jang
Jul 18, 2017·Analytical Biochemistry·Ljubava D ZorovaDmitry B Zorov
Dec 13, 2017·Cell Death and Differentiation·Niamh M C ConnollyJochen H M Prehn
Sep 11, 2018·Frontiers in Oncology·Giuseppe CanninoAndrea Rasola
Oct 21, 2018·Journal of Immunological Methods·Lina ChakrabartiPamela Hawley-Nelson
Apr 6, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Charleston F ChristieEduardo N Maldonado
May 30, 2019·Frontiers in Oncology·Monica NeaguAristidis M Tsatsakis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.