Quantitative Analysis of Mouse Retinal Layers Using Automated Segmentation of Spectral Domain Optical Coherence Tomography Images

Translational Vision Science & Technology
Chantal DysliMartin S Zinkernagel

Abstract

Quantification of retinal layers using automated segmentation of optical coherence tomography (OCT) images allows for longitudinal studies of retinal and neurological disorders in mice. The purpose of this study was to compare the performance of automated retinal layer segmentation algorithms with data from manual segmentation in mice using the Spectralis OCT. Spectral domain OCT images from 55 mice from three different mouse strains were analyzed in total. The OCT scans from 22 C57Bl/6, 22 BALBc, and 11 C3A.Cg-Pde6b(+)Prph2(Rd2) /J mice were automatically segmented using three commercially available automated retinal segmentation algorithms and compared to manual segmentation. Fully automated segmentation performed well in mice and showed coefficients of variation (CV) of below 5% for the total retinal volume. However, all three automated segmentation algorithms yielded much thicker total retinal thickness values compared to manual segmentation data (P < 0.0001) due to segmentation errors in the basement membrane. Whereas the automated retinal segmentation algorithms performed well for the inner layers, the retinal pigmentation epithelium (RPE) was delineated within the sclera, leading to consistently thicker measurements of t...Continue Reading

Citations

Oct 26, 2016·Experimental Eye Research·Chantal DysliVolker Enzmann
Apr 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Elliott H SohnMichael David Abràmoff
Feb 16, 2017·Scientific Reports·Marci L DeRamusSteven J Pittler
Aug 2, 2019·Investigative Ophthalmology & Visual Science·Petr SoukupSimon Pot
Feb 8, 2020·Investigative Ophthalmology & Visual Science·Da ZhaoBang V Bui
Feb 14, 2020·Investigative Ophthalmology & Visual Science·Kore ChanGillian J McLellan
Feb 23, 2020·Circulation Research·Jee Myung YangInjune Kim
Nov 20, 2016·Medicine·Jae Yon WonYoung-Hoon Park
May 23, 2019·Frontiers in Neuroscience·Viviana Rincón MontesAndreas Offenhäusser
Sep 25, 2019·Indian Journal of Ophthalmology·Gunjan DeshpandeRicha Gupta
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Feb 6, 2021·Frontiers in Aging Neuroscience·Elena Salobrar-GarcíaJosé M Ramírez
Jan 8, 2021·Disease Models & Mechanisms·Rachel AtkinsonAnna King
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Jul 6, 2021·Frontiers in Immunology·Lydia J BradleyLindsay B Nicholson
Jul 24, 2021·Visual Neuroscience·Andrey V DmitrievRobert A Linsenmeier

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