Quantitative analysis of multiple V-region interactions among normal human IgG

European Journal of Immunology
A AyoubaA Coutinho

Abstract

There is to date no quantitative method for scoring putative V-region interactions among serum antibodies, and the available qualitative techniques are not amenable to routine utilization. This deficit may explain the paucity of observations on the characteristics of the immune network, in contrast with the multiplicity of phenomenological descriptions on idiotype regulation. We describe here a novel methodology that uses isoelectric focusing (IEF) to resolve human F(ab')2 preparations from large pools of normal serum IgG into multiple bands, and computer-aided data processing to analyze interactions between the resulting blotted proteins and normal serum IgG from individual donors. Our results show that in all normal human sera tested, there are IgG-mediated interactions with a large number of IEF fractions of human F(ab')2. These interactions are V region specific, as assessed by inhibition experiments and by lack of binding of IgG monoclonal antibodies, and are characterized by average affinities that are in the micromolar range, as measured by surface plasmon resonance.

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Citations

Jun 16, 2000·Journal of Immunological Methods·D StahlM D Kazatchkine
Oct 6, 1998·Journal of Immunological Methods·S Lacroix-DesmazesM D Kazatchkine
Apr 29, 1999·Scandinavian Journal of Immunology·U Pacheco-GarcíaF J Sánchez-García
Dec 19, 2000·Scandinavian Journal of Immunology·L Padierna-OlivosF J Sánchez-García
Apr 20, 2001·Scandinavian Journal of Immunology·T Tovar-RiveraF J Sánchez-García
Sep 7, 2007·Annals of the New York Academy of Sciences·Natalia Pavón-MartínezVictor Manuel Monteón
Aug 14, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Iglika Djoumerska-AlexievaAndrey Tchorbanov

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