Quantitative analysis of PEG-functionalized colloidal gold nanoparticles using charged aerosol detection

Analytical and Bioanalytical Chemistry
Mackensie C SmithScott E McNeil

Abstract

Surface characteristics of a nanoparticle, such as functionalization with polyethylene glycol (PEG), are critical to understand and achieve optimal biocompatibility. Routine physicochemical characterization such as UV-vis spectroscopy (for gold nanoparticles), dynamic light scattering, and zeta potential are commonly used to assess the presence of PEG. However, these techniques are merely qualitative and are not sensitive enough to distinguish differences in PEG quantity, density, or presentation. As an alternative, two methods are described here which allow for quantitative measurement of PEG on PEGylated gold nanoparticles. The first, a displacement method, utilizes dithiothreitol to displace PEG from the gold surface. The dithiothreitol-coated gold nanoparticles are separated from the mixture via centrifugation, and the excess dithiothreitol and dissociated PEG are separated through reversed-phase high-performance liquid chromatography (RP-HPLC). The second, a dissolution method, utilizes potassium cyanide to dissolve the gold nanoparticles and liberate PEG. Excess CN(-), Au(CN)2 (-), and free PEG are separated using RP-HPLC. In both techniques, the free PEG can be quantified against a standard curve using charged aerosol de...Continue Reading

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Citations

Mar 15, 2016·International Journal of Pharmaceutics·George Dan MogoşanuLudovic Everard Bejenaru
Sep 11, 2016·The AAPS Journal·Jennifer H GrossmanJeffrey D Clogston
May 6, 2019·Electrophoresis·Siyao Liu, Michael Lämmerhofer
Feb 3, 2020·Journal of Chromatography. a·Klaus Schilling, Ulrike Holzgrabe

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