Quantitative analysis of plasma cell-free Epstein-Barr virus DNA in nasopharyngeal carcinoma after salvage nasopharyngectomy: a prospective study

Head & Neck
William I WeiJonathan S T Sham

Abstract

The quantity of circulating cell-free Epstein-Barr virus (EBV) DNA in patients with nasopharyngeal carcinoma (NPC) managed by radiotherapy has prognostic relevance. We measured the copy number of EBV DNA in patients with early recurrent NPC before and after salvage nasopharyngectomy. Nasopharyngectomy with the maxillary swing approach was performed for 28 patients. Serum blood samples were taken prospectively before nasopharyngectomy and on postoperative day 7. Plasma cell-free EBV DNA copies were measured with a real-time quantitative polymerase chain reaction for the BamHI-W fragment of the EBV genome. Cell-free EBV DNA was detected in 17 patients before nasopharyngectomy. Surgical resection reduced the copy number of EBV DNA significantly (p = .016). Negative surgical margins achieved during nasopharyngectomy is associated with a zero EBV DNA copy postoperatively (p = .022). Cell-free EBV DNA was detected in 61% of patients with recurrent NPC, and its quantity postoperatively reflects whether the salvage nasopharyngectomy has achieved a negative surgical margin.

References

Apr 29, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M Al-SarrafJ F Ensley
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Mar 12, 2002·Archives of Otolaryngology--head & Neck Surgery·Willard E FeeDon Goffinet

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Citations

Jun 14, 2005·Lancet·William I Wei, Jonathan S T Sham
Mar 20, 2012·Oral Oncology·Anne W M LeeL K Chan
Aug 18, 2005·International Journal of Cancer. Journal International Du Cancer·Xiaohong YangAllan Hildesheim
Dec 2, 2006·Biochimica Et Biophysica Acta·M Fleischhacker, B Schmidt
Sep 13, 2005·International Journal of Oral and Maxillofacial Surgery·R Shaw
Apr 10, 2010·Clinical and Experimental Otorhinolaryngology·William I Wei, Dora L W Kwong
Jul 5, 2006·Biological & Pharmaceutical Bulletin·Enver AltasYavuz Sutbeyaz
Sep 24, 2018·Journal of Ovarian Research·Katarina KalavskaMichal Mego
Jun 22, 2021·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Anne W M LeeAlfio Ferlito

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