Quantitative analysis of pulmonary surfactant phospholipids by high-performance liquid chromatography and light-scattering detection

Journal of Chromatography. B, Biomedical Applications
H Bünger, U Pison

Abstract

An improved high-performance liquid chromatographic method for the separation and quantitation of nine phospholipid classes is described. It is based on normal-phase chromatography with silica gel as stationary phase and a binary gradient with mixtures of chloroform, methanol and water as mobile phase. The response of the evaporative light-scattering detector was non-linear. Peak areas were proportional to the power 1.7 of the masses. Phospholipids in lung lavage samples were enriched by liquid extraction prior to HPLC analysis. The described method is a rapid and accurate procedure for the quantitative analysis of phospholipid classes in biological samples.

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Citations

Aug 29, 2013·Lipids·Francesca GiuffridaMarcel Braun
May 26, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Laura Rabinovich-GuilattPierre Chaminade
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Sep 3, 2005·Biomedical Chromatography : BMC·Brianna L Peterson, Brian S Cummings
Dec 4, 2012·Biochimica Et Biophysica Acta·Victoria GossAnthony D Postle
Sep 19, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Laurence Zanna, Jean-François Haeuw
Apr 23, 2004·Journal of Lipid Research·Nicolas MazzellaJean-Claude Bertrand
Mar 21, 2007·Electrophoresis·Fei GaoHuwei Liu
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Dec 19, 2018·Biomolecules·Spiro KhouryJustine Bertrand-Michel
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Jan 10, 2013·Journal of Agricultural and Food Chemistry·Bertram FongCarmen Norris

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