Quantitative analysis of redox-sensitive proteome with DIGE and ICAT.

Journal of Proteome Research
Cexiong FuHong Li

Abstract

Oxidative modifications of protein thiols are important mechanisms for regulating protein functions. The present study aimed to compare the relative effectiveness of two thiol-specific quantitative proteomic techniques, difference gel electrophoresis (DIGE) and isotope coded affinity tag (ICAT), for the discovery of redox-sensitive proteins in heart tissues. We found that these two methods were largely complementary; each could be used to reveal a set of unique redox-sensitive proteins. Some of these proteins are low-abundant signaling proteins and membrane proteins. From DIGE analysis, we found that both NF-kappaB-repressing protein and epoxide hydrolase were sensitive to H 2O 2 oxidation. In ICAT analysis, we found that specific cysteines within sacroplasmic endoplamic reticulum calcium ATPase 2 and voltage-dependent anion-selective channel protein 1 were sensitive to H 2O 2 oxidation. From these analyses, we conclude that both methods should be employed for proteome-wide studies, to maximize the possibility of identifying proteins containing redox-sensitive cysteinyl thiols in complex biological systems.

References

May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M B Toledano, W J Leonard
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·S XanthoudakisT Curran
Jul 1, 1995·Molecular and Cellular Biology·R RainwaterK Mann
Sep 2, 1996·FEBS Letters·H WoloskerS Engelender
Jun 15, 2000·The Journal of Biological Chemistry·E CabiscolJ Ros
Aug 5, 2000·Free Radical Biology & Medicine·Y M Janssen-HeiningerP A Baeuerle
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·H YanoB B Buchanan
Feb 22, 2002·Japanese Journal of Pharmacology·Toshio Obata
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Maddalena FratelliPietro Ghezzi
Aug 22, 2002·The Journal of Biological Chemistry·Kenneth M HumphriesSusan S Taylor
Dec 12, 2002·Cellular and Molecular Life Sciences : CMLS·P C F Graf, U Jakob
Jun 13, 2003·Archives of Biochemistry and Biophysics·Vadim V Sumbayev
Jul 23, 2003·Proteomics·Pietro Ghezzi, Valentina Bonetto
Dec 9, 2003·Amino Acids·P Di SimplicioD Di Giuseppe
Dec 12, 2003·The Journal of Biological Chemistry·Hee-Sae ParkEui-Ju Choi
Dec 31, 2003·The Journal of Cell Biology·Yun Li, Patricia Camacho
Jan 17, 2004·Molecular & Cellular Proteomics : MCP·Mahadevan SethuramanRichard A Cohen
Jun 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rosa M Canet-AvilésMark R Cookson
Oct 27, 2004·PLoS Biology·Lars I Leichert, Ursula Jakob
Nov 13, 2004·The Biochemical Journal·Pedro IñarreaEnrique Cadenas
Dec 18, 2004·The Biochemical Journal·Yoko NagumoHiroyuki Osada
Dec 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Adrian T SaurinPhilip Eaton
May 17, 2005·Brain Research. Molecular Brain Research·Keith H ChristoffersRichard D Howells
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ana P VivancosElena Hidalgo
Nov 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Laure MicheletStéphane D Lemaire
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Gang HaoSteven S Gross
Aug 22, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Arti B SharmaRobert T Mallet
Mar 28, 2007·Journal of Neuroscience Research·Shelley F NewmanD Allan Butterfield

❮ Previous
Next ❯

Citations

Apr 20, 2010·Amino Acids·Corinne M Spickett, Andrew R Pitt
Dec 17, 2010·Journal of Proteome Research·Xin HuangShi-Jian Ding
Aug 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Junya KurodaJunichi Sadoshima
Jun 1, 2012·Molecular & Cellular Proteomics : MCP·Pablo Martínez-AcedoJesús Vázquez
May 7, 2009·Molecular & Cellular Proteomics : MCP·Cexiong FuHong Li
Feb 1, 2011·Antioxidants & Redox Signaling·Marika LindahlThomas Kieselbach
May 23, 2012·Antioxidants & Redox Signaling·Vikas KumarMarkus Michael Bachschmid
Sep 2, 2010·Cardiovascular Research·Marjan Gucek, Elizabeth Murphy
Feb 1, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Mark J KohrCharles Steenbergen
Oct 18, 2012·Circulation. Cardiovascular Genetics·Christopher I Murray, Jennifer E Van Eyk
Oct 22, 2009·American Journal of Respiratory Cell and Molecular Biology·Page C SpiessAlan R Buckpitt
Jan 1, 2009·Human Genomics and Proteomics : HGP·Kondethimmanahalli Chandramouli, Pei-Yuan Qian
Mar 19, 2013·Future Medicinal Chemistry·Pedro L Zamora, Frederick A Villamena
Apr 20, 2014·Nature Protocols·Sarela García-SantamarinaElena Hidalgo
Dec 18, 2013·Trends in Plant Science·Mark Stitt, Yves Gibon
Jan 19, 2011·Journal of Proteomics·Angelo D'AlessandroLello Zolla
Oct 18, 2011·Expert Review of Proteomics·Kevin M Coombs
Jan 18, 2012·Expert Opinion on Drug Metabolism & Toxicology·Claudia V PereiraYvonne Will
Jan 20, 2016·Biochimica Et Biophysica Acta·Hans-Peter Mock, Karl-Josef Dietz
Nov 7, 2015·Journal of Proteome Research·Zhe QuZezong Gu
Oct 20, 2010·Journal of Molecular and Cellular Cardiology·Shouji MatsushimaJunichi Sadoshima
Nov 5, 2011·Journal of Proteome Research·Hai WangYiji Xia
May 15, 2013·Proteomics. Clinical Applications·Onno B BleijerveldDominique P V de Kleijn
Jan 20, 2011·Proteomics·Laurence V Bindschedler, Rainer Cramer
Mar 4, 2014·The Plant Journal : for Cell and Molecular Biology·Mengmeng ZhuSixue Chen
Jun 17, 2014·Mass Spectrometry Reviews·D Allan ButterfieldRenã A S Robinson
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Mark StittBjörn Usadel
Jun 15, 2010·Environmental and Molecular Mutagenesis·James R Roede, Dean P Jones
Feb 23, 2013·Methods : a Companion to Methods in Enzymology·Changgong WuHong Li
Apr 30, 2013·Biochimica Et Biophysica Acta·Rebecca CharlesPhilip Eaton
Jul 18, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Joseph L JilekCraig Harris
Oct 28, 2015·Horticulture Research·Kelly Mayrink BalmantSixue Chen
Feb 5, 2013·Free Radical Biology & Medicine·Vikas KumarChristine C Winterbourn
Feb 19, 2013·Biochimica Et Biophysica Acta·Pietro Ghezzi
May 1, 2013·Methods : a Companion to Methods in Enzymology·Yi-Ju ChenYu-Ju Chen
Dec 7, 2010·Current Opinion in Chemical Biology·Edward T ChouchaniMichael P Murphy
Dec 7, 2010·Current Opinion in Chemical Biology·Maike Thamsen, Ursula Jakob

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.