Jul 1, 1988

Quantitative analysis of RNA produced by slow and fast alleles of Adh in Drosophila melanogaster

Proceedings of the National Academy of Sciences of the United States of America
C C Laurie, L F Stam

Abstract

The alcohol dehydrogenase (ADH) locus (Adh) of Drosophila melanogaster in polymorphic on a world-wide basis for two allozymes, Fast and Slow. This study was undertaken to determine whether the well-established difference in ADH protein concentration between the allozymes is due to a difference in mRNA levels. RNA gel blot hybridization and an RNase protection assay were used to quantify ADH mRNA levels. Each method used an Adh null mutant as an internal standard. Several Slow and Fast allele pairs of different geographic origins were analyzed. The results provide strong evidence that the ADH protein concentration difference is not accounted for by RNA level.

  • References18
  • Citations49

Mentioned in this Paper

Transformation, Genetic
Nucleic Acid Hybridization Procedure
Alcoholic Intoxication, Chronic
RNase Protection Assay
Alkaline Ribonuclease
Alcohol Dehydrogenase I
Mutant
Genomic Hybridization
Alcohol dehydrogenase
Locus

About this Paper

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