Quantitative analysis of serum-based IgG agalactosylation for tuberculosis auxiliary diagnosis.

Glycobiology
Peng LiuXiao-Lian Zhang

Abstract

Tuberculosis (TB) is the leading infectious cause of mortality worldwide, especially in developing countries. However, effective means for TB diagnosis, especially for bacillus-negative (Bn) TB laboratory diagnosis, are urgently needed. In the present study, serum IgG from each tuberculosis patients and healthy controls was purified using affinity chromatography. The samples were then analyzed using mass spectrometry (MS) and ultraperformance liquid chromatography (UPLC) methods. We quantitatively assessed the changes of serum IgG galactosylation in 567 human serum samples including 377 pulmonary TB patients and 190 healthy donors (HDs). We found significantly more agalactosylated (G0) vs monogalactosylated (G1) and digalactosylated (G2) N-glycans of IgG in TB patients, including smear-negative TB patients, than in HDs. The detection rate of TB diagnostic performance by MS for IgG-Gal ratio G0/(G1 + G2 × 2) is 90.48% for bacillus-positive (Bp) and 73.16% for Bn TB patients. Further, combination of MS method with other routine laboratory TB diagnostic methods significantly increased the detection rate to 91.01%-98.39%. Similar results were observed in Mycobacterium tuberculosis (M. tb) infection mouse models. The decrease in gal...Continue Reading

References

Mar 1, 1992·The Journal of Clinical Investigation·J S AxfordI M Roitt
Jun 3, 1988·Science·J A Swets
Jul 1, 1993·Rapid Communications in Mass Spectrometry : RCM·D J Harvey
May 3, 2003·The Lancet Infectious Diseases·Kamran SiddiqiJohn Walley
Apr 3, 2009·Clinical and Vaccine Immunology : CVI·Xinchun ChenBoping Zhou
Feb 13, 2010·Cellular Microbiology·Bibhuti B MishraElsa Anes
Aug 8, 2013·Analytical Chemistry·Henning StöckmannPauline M Rudd
Mar 20, 2014·Cancer Biomarkers : Section a of Disease Markers·Simone AlbrechtPauline M Rudd
Jul 28, 2016·Statistics in Medicine·Ashutosh R TamhaneGary R Cutter
Sep 27, 2016·Cell·Lenette L LuGalit Alter
Oct 28, 2016·Psychonomic Bulletin & Review·Ronald A Rensink
Aug 15, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·January WeinerJayne S Sutherland
Oct 31, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Paulo S Silveira-MattosBruno B Andrade

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Chonnam Medical Journal
Fariz NurwidyaFaisal Yunus
Frontiers in Microbiology
Muhammad Tahir KhanDong-Qing Wei
Zeitschrift für Gerontologie und Geriatrie
S Rajagopalan, T T Yoshikawa
© 2021 Meta ULC. All rights reserved