Quantitative analysis of small molecules by desorption electrospray ionization mass spectrometry from polytetrafluoroethylene surfaces

Rapid Communications in Mass Spectrometry : RCM
Demian R IfaR Graham Cooks

Abstract

Desorption electrospray ionization mass spectrometry (DESI-MS) is an emerging technique for ambient analysis. However, its application to routine quantitation has not been explored extensively and this is undertaken here. We present studies that utilize a particularly suitable surface, porous polytetrafluoroethylene (porous PTFE), which shows less cross contamination between samples and improved sensitivity and signal stability compared to other surfaces. Quantitative experiments for 1 microL spotted solutions of the beta-blocker propranolol, using isotopically labeled propranolol as internal standard, showed a good linear correlation (r2 > 0.996) over the range 0.01-100 microM. The inter-day precision, based on the relative standard deviation, for replicates analyzed on three different days was 13% for 0.01 microM and better than 7% for the remainder of the calibration points. The inter-day accuracy, expressed as relative error, was better than +/-7% for all calibration points along the curve. These day-to-day measurements suggest that DESI-MS can be successfully employed for routine quantitative analysis. The use of the analog atenolol as an internal standard and further considerations that should improve quantitation by DESI...Continue Reading

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Citations

Feb 26, 2009·Analytical and Bioanalytical Chemistry·Demian R IfaR Graham Cooks
Jun 4, 2010·Analytical and Bioanalytical Chemistry·Rosana M AlbericiMarcos N Eberlin
Apr 6, 2011·Analytical and Bioanalytical Chemistry·Andrea PennaGiovanni Predieri
Jul 27, 2011·Analytical and Bioanalytical Chemistry·Allison L DillR Graham Cooks
Aug 22, 2012·Journal of the American Society for Mass Spectrometry·Kevin A DouglassAndre R Venter
Apr 6, 2013·Journal of the American Society for Mass Spectrometry·Ruixia ShiJin Ouyang
Apr 23, 2013·Journal of the American Society for Mass Spectrometry·Elaine C CabralDemian R Ifa
Aug 8, 2008·Journal of the American Society for Mass Spectrometry·Joseph E Chipuk, Jennifer S Brodbelt
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