Quantitative analysis of the calcium-sensing receptor messenger RNA in parathyroid adenomas

Surgery
S C GarnerL D Quarles

Abstract

In primary hyperparathyroidism, hypercalcemia fails to suppress adequately secretion of parathyroid hormone by the parathyroid gland, which may result from failure of the cell-surface calcium receptor (CaR) to sense calcium correctly. Quantification of mRNA concentrations should provide important information on the role of expression of Call in primary hyperparathyroidism. We have developed a quantitative reverse transcriptase-polymerase chain reaction assay with a competitive template (CaR-M). Amplified cDNAs for CaR and CaR-M are quantified, and the concentration of CaR mRNA is determined from the ratio of CaR-M/CaR versus known CaR-M concentrations. In parathyroid adenomas (n = 12) the CaR mRNA was 19.2 +/- 2.4 (mean +/- SE) fg/ng total RNA (range, 7.4 to 32.8 fg/ng). Extracellular ionized calcium levels ranged from 1.38 to 1.74 mmol/L (normal 1.19 to 1.31 mmol/L) and parathyroid hormone from 69 to 345 pg/ml (normal, 14 to 65 pg/ml). In spite of the wide variability in CaR expression in the various adenomas, there was no correlation between mRNA and either extracellular ionized calcium (r2 = 0.013) parathyroid hormone levels (r2 = 0.001). Normal human parathyroid glands gave values of 8.0 and 16.6 fg/ng, whereas normal bovin...Continue Reading

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Citations

Oct 18, 2000·Endocrinology and Metabolism Clinics of North America·E M Brown
Nov 23, 2006·Arquivos brasileiros de endocrinologia e metabologia·Lília D'Souza-Li
Jul 2, 2020·The Journal of Clinical Endocrinology and Metabolism·Priyanka SinghSudhaker Dhanwada Rao
Sep 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·R GuoL D Quarles
Jan 12, 2001·Physiological Reviews·E M Brown, R J MacLeod
Feb 9, 2005·Critical Reviews in Clinical Laboratory Sciences·Jacob Tfelt-Hansen, Edward M Brown

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