Quantitative analysis of the immune response upon Salmonella typhimurium infection along the porcine intestinal gut.

Veterinary Research
Melania Collado-RomeroJuan J Garrido

Abstract

Salmonella enterica serovar Typhimurium causes enteric disease and compromises food safety. In pigs, the molecular response of the intestine to S. typhimurium has been traditionally characterized by in vitro models that do not reflect the actual immunological competence of the intestinal mucosa. In this work, we performed an oral S. typhimurium infection study to obtain insight into the in vitro response in three different sections (jejunum, ileum and colon) of the porcine intestine. For this, samples from one-month-old infected piglets were collected during a time course comprising 1, 2, and 6 days post inoculation to evaluate the intestinal response by quantifying the mRNA expression of gene coding for 28 innate immune system molecules using quantitative real-time PCR assays. In addition, samples from non-infected control animals were also employed to establish differences in the steady state gene expression between intestinal sections. The panel of quantified molecules included an assortment of cytokines, chemokines, pattern-recognition receptors, intracellular signaling molecules, transcription factors and antimicrobial molecules. Changes in gene expression occurred in the three different parts of the intestine and during t...Continue Reading

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Citations

Jun 20, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Elisabetta RazzuoliMassimo Amadori
Dec 19, 2012·Virology Journal·Mia Madel AlfajaroKyoung-Oh Cho
Dec 10, 2013·BMC Veterinary Research·Mackenzie J SlifierzAbdolvahab Farzan
Nov 10, 2012·Journal of Animal Science and Biotechnology·Shuhong ZhaoHongbo Chen
Mar 1, 2014·Animal : an International Journal of Animal Bioscience·M J SlifierzA Farzan
Oct 9, 2015·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Cinzia ZanottiMassimo Amadori
Sep 22, 2015·Frontiers in Cellular and Infection Microbiology·Melania Collado-RomeroJuan J Garrido
May 21, 2019·Veterinary Pathology·Natividad Bellido-CarrerasJuan J Garrido
Nov 16, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Martine Schroyen, Christopher K Tuggle
Jan 27, 2017·Helicobacter·Iris BosschemFreddy Haesebrouck
Jul 11, 2018·Journal of Animal Science and Biotechnology·Chuanjian ZhangWeiyun Zhu
Jan 18, 2021·Research in Veterinary Science·José M FloresMaría Dolores Hernando

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Datasets Mentioned

BETA
M86923

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
scraping
electrophoresis

Software Mentioned

BestKeeper
SPSS
BLAST

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