Quantitative Analysis of the Physiological Heterogeneity within Starved Cultures of Micrococcus luteus by Flow Cytometry and Cell Sorting.

Applied and Environmental Microbiology
Arseny S KaprelyantsDouglas B Kell

Abstract

A high proportion of Micrococcus luteus cells in cultures which had been starved for 3 to 6 months lost the ability to grow and form colonies on agar plates but could be resuscitated from their dormancy by incubation in an appropriate liquid medium (A. S. Kaprelyants and D. B. Kell, Appl. Environ. Microbiol. 59:3187-3196, 1993). We used flow cytometry and cell sorting to study populations of bacteria that had been starved for 5 months. These cells could be stained by the fluorescent lipophilic cation rhodamine 123, but such staining was almost independent of metabolically generated energy in that it was not affected by uncouplers. Two populations could be distinguished, one with a lower degree of rhodamine fluorescence (a degree of fluorescence referred to as region A and containing approximately 80% of the cells) and one with a more elevated degree of fluorescence (region B, approximately 20% of the cells). Subsequent incubation of starved cells in fresh medium in the presence of the antibiotic chloramphenicol (to which M. luteus is sensitive) resulted in the transient appearance of cells actively accumulating rhodamine 123 (and fluorescing in region B) and of larger cells exhibiting a yet-greater degree of fluorescence (regio...Continue Reading

References

Dec 15, 1992·FEMS Microbiology Letters·D WeichartS Kjelleberg
Jun 1, 1991·Applied and Environmental Microbiology·J A MorganR W Pickup
Jan 1, 1995·Critical Reviews in Biotechnology·G J Salter, D B Kell
Apr 1, 1993·FEMS Microbiology Reviews·A S KaprelyantsD B Kell
Mar 1, 1995·Trends in Ecology & Evolution·D B KellA Grafen

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Citations

Jul 14, 2001·FEMS Microbiology Ecology·J StenströmM Johansson
Nov 10, 2012·Applied Microbiology and Biotechnology·Julián Rafael DibFriedhelm Meinhardt
Apr 15, 2020·Current Protocols in Cytometry·Hazel Davey, Stéphane Guyot
Sep 5, 2008·Current Protocols in Cytometry·Hazel M DaveyArseny S Kaprelyants
Sep 19, 2008·Applied Microbiology and Biotechnology·Aline RaultCatherine Béal
Jun 13, 2009·Journal of Food Protection·Alissa M WescheElliot T Ryser
Jul 31, 2013·Orvosi hetilap·Eva Pállinger
May 10, 2017·Journal of Bacteriology·Sujina MaliSteven J Bark
Sep 5, 2008·Current Protocols in Cytometry·Jonathan Porter
Jun 28, 2011·Applied and Environmental Microbiology·Hazel M Davey
Jun 25, 2013·Theory in Biosciences = Theorie in Den Biowissenschaften·Vic NorrisJean-Nicolas Audinot
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Vic NorrisKirsten Skarstad
May 6, 2015·FEMS Microbiology Reviews·Marnie PotgieterEtheresia Pretorius
Sep 25, 2019·Journal of Bacteriology·Spencer HavisSteven J Bark
Aug 1, 1998·Microbiology·Michael R BarerDouglas B Kell

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