PMID: 6257505Oct 1, 1980

Quantitative analysis of the proton and charge stoichiometry of cytochrome c oxidase from beef heart reconstituted into phospholipid vesicles

European Journal of Biochemistry
E Sigel, E Carafoli


The proton and charge stoichiometry of cytochrome c oxidase reconstituted into phospholipid vesicles has been analysed using a fast responding oxygen electrode and a system for the simultaneous measurement of H+, K+ or lipophilic cations, and O2. From initial rate measurements after addition of reductant (ascorbate /N,N,N',N'-tetramethyl-p-phenylenediamine) the following stoichiometries could be extrapolated: K+/e-, between 1.85 and 2.05, H+/e-, between 0.58 and 0.75. Lipophilic cations can replace valinomycin/potassium as the charge . compensating system. Net H+ extrusion was observed for up to 11 turnovers of the enzyme (11 O2/cytochrome aa3). The variation of the internal pH buffering capacity of the vesicles has an influence on the number of turnovers during which net proton translocation can be observed, but has no influence on the determined stoichiometries.


Aug 15, 1978·European Journal of Biochemistry·E Sigel, E Carafoli
Oct 11, 1978·Biochimica Et Biophysica Acta·K Krab, M Wikström
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A AlexandreA L Lehninger
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T PozzanG F Azzone
Apr 27, 1979·Biochemical and Biophysical Research Communications·R P CaseyA Azzi
Aug 17, 1979·Biochimica Et Biophysica Acta·M Wikström, K Krab
Oct 10, 1979·Biochimica Et Biophysica Acta·K Krab, M Wikström
Apr 30, 1976·Biochimica Et Biophysica Acta·S Papa
Nov 17, 1977·Biochimica Et Biophysica Acta·M Wikström, H T Saari
Apr 15, 1978·FEBS Letters·J Moyle, P Mitchell
Jan 2, 1975·Biochemical and Biophysical Research Communications·V M Madeira
Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell
Feb 25, 1980·FEBS Letters·S PapaN Altamura
Jan 1, 1977·Annual Review of Biochemistry·P D BoyerE C Slater


Apr 1, 1987·Journal of Bioenergetics and Biomembranes·L J Prochaska, P S Fink
Oct 1, 1991·Journal of Bioenergetics and Biomembranes·M T Wilson, D Bickar
Jan 1, 1984·Progress in Biophysics and Molecular Biology·G Benga, R P Holmes
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B ReynafarjeA L Lehninger
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G AntoniniM Brunori
Nov 16, 1987·European Journal of Biochemistry·M BrunoriM T Wilson
Jul 11, 2006·Biochimica Et Biophysica Acta·Giorgio LenazAnnalisa Biondi
Apr 9, 1991·Biochemistry·P C HinkleD L Harris
May 15, 1989·Biochemical and Biophysical Research Communications·D Steverding, B Kadenbach
Jun 15, 1983·European Journal of Biochemistry·T Penttilä
Jul 1, 1981·European Journal of Biochemistry·F Di VirgilioG F Azzone

Related Concepts

Bos indicus
Cytochrome c Oxidase Subunit VIa
Hydrogen-Ion Concentration
Oxygen Consumption

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.