PMID: 6257505Oct 1, 1980

Quantitative analysis of the proton and charge stoichiometry of cytochrome c oxidase from beef heart reconstituted into phospholipid vesicles

European Journal of Biochemistry
E Sigel, E Carafoli

Abstract

The proton and charge stoichiometry of cytochrome c oxidase reconstituted into phospholipid vesicles has been analysed using a fast responding oxygen electrode and a system for the simultaneous measurement of H+, K+ or lipophilic cations, and O2. From initial rate measurements after addition of reductant (ascorbate /N,N,N',N'-tetramethyl-p-phenylenediamine) the following stoichiometries could be extrapolated: K+/e-, between 1.85 and 2.05, H+/e-, between 0.58 and 0.75. Lipophilic cations can replace valinomycin/potassium as the charge . compensating system. Net H+ extrusion was observed for up to 11 turnovers of the enzyme (11 O2/cytochrome aa3). The variation of the internal pH buffering capacity of the vesicles has an influence on the number of turnovers during which net proton translocation can be observed, but has no influence on the determined stoichiometries.

References

Aug 15, 1978·European Journal of Biochemistry·E Sigel, E Carafoli
Oct 11, 1978·Biochimica Et Biophysica Acta·K Krab, M Wikström
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A AlexandreA L Lehninger
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T PozzanG F Azzone
Apr 27, 1979·Biochemical and Biophysical Research Communications·R P CaseyA Azzi
Aug 17, 1979·Biochimica Et Biophysica Acta·M Wikström, K Krab
Oct 10, 1979·Biochimica Et Biophysica Acta·K Krab, M Wikström
Apr 30, 1976·Biochimica Et Biophysica Acta·S Papa
Nov 17, 1977·Biochimica Et Biophysica Acta·M Wikström, H T Saari
Apr 15, 1978·FEBS Letters·J Moyle, P Mitchell
Jan 2, 1975·Biochemical and Biophysical Research Communications·V M Madeira
Aug 1, 1966·Biological Reviews of the Cambridge Philosophical Society·P Mitchell
Feb 25, 1980·FEBS Letters·S PapaN Altamura
Jan 1, 1977·Annual Review of Biochemistry·P D BoyerE C Slater

Citations

Apr 1, 1987·Journal of Bioenergetics and Biomembranes·L J Prochaska, P S Fink
Oct 1, 1991·Journal of Bioenergetics and Biomembranes·M T Wilson, D Bickar
Jan 1, 1984·Progress in Biophysics and Molecular Biology·G Benga, R P Holmes
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B ReynafarjeA L Lehninger
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G AntoniniM Brunori
Nov 16, 1987·European Journal of Biochemistry·M BrunoriM T Wilson
Jul 11, 2006·Biochimica Et Biophysica Acta·Giorgio LenazAnnalisa Biondi
Apr 9, 1991·Biochemistry·P C HinkleD L Harris
May 15, 1989·Biochemical and Biophysical Research Communications·D Steverding, B Kadenbach
Jun 15, 1983·European Journal of Biochemistry·T Penttilä
Jul 1, 1981·European Journal of Biochemistry·F Di VirgilioG F Azzone

Related Concepts

Bos indicus
Cytochrome c Oxidase Subunit VIa
Hydrogen-Ion Concentration
Niosomes
Myocardium
Oxygen Consumption
Phospholipids
Potassium

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