Quantitative analysis of the redox states of cytochromes in a living L929 (NCTC) cell by resonance Raman microspectroscopy

Journal of Biophotonics
Minoru KakitaHiro-o Hamaguchi

Abstract

Raman spectra and images of a living L929 (NCTC) cell have been measured with 532 nm excitation. Both reduced and oxidized forms of cytochromes b and c (cyt b and cyt c) have been observed in situ without any pretreatment. The redox states of cyts b and c have been assessed quantitatively with a spectral analysis. It has been found that reduced cyt c is more abundant than oxidized cyt c, while oxidized cyt b is slightly more abundant than reduced cyt b in a living cell.

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Citations

Dec 3, 2014·Journal of Photochemistry and Photobiology. B, Biology·Konstantin A Okotrub, Nikolay V Surovtsev
Dec 5, 2015·Biophysical Journal·Konstantin A Okotrub, Nikolay V Surovtsev
Jun 19, 2013·Current Opinion in Chemical Biology·Almar F PalonponKatsumasa Fujita
Nov 28, 2014·Analytical and Bioanalytical Chemistry·Christoph Krafft, Jürgen Popp
Jan 11, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hemanth NoothalapatiTatsuyuki Yamamoto
Apr 29, 2018·European Biophysics Journal : EBJ·Konstantin A Okotrub, Nikolay V Surovtsev
Dec 1, 2018·Metallomics : Integrated Biometal Science·Theodora J Stewart
Jan 14, 2021·Journal of Biophotonics·Joshua W LalondeMichael L Denton

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