PMID: 15348014Sep 7, 2004Paper

Quantitative analysis of the regulation of leukocyte chemosensory migration by a vascular prosthetic biomaterial

Journal of Materials Science. Materials in Medicine
C C ChangPrabhas V Moghe

Abstract

The need for improved, infection-resistant vascular biomaterials calls for more objective evaluation of the immune pathophysiology of implantable prosthetic materials. In this study we have developed a new strategy to quantitatively characterize population-averaged responses of immune cell migration on vascular prosthetic materials. This approach, incorporating a chemokinetically regulated "biomaterial-gel" sandwich configuration, was applied to quantify both random and directed modes of the chemosensory migration of human neutrophil leukocytes on expanded polytetrafluoroethylene (ePTFE). Our studies show that (a) microporous, synthetic materials like ePTFE suppress the basal rate of random cell migration relative to that reported on non-porous control surfaces; (b) stimulation with chemoattractant (formyl peptide) can significantly elevate rates of random and directed migration on ePTFE; and (c) protein conditioning of ePTFE with albumin or immunoglobulin G can differentially modulate the rates and relative proportion of random and directional components of leukocyte migration response to chemoattractant. This, to our knowledge, is the first objective quantitation of chemokinetically regulated cell migration on implantable pro...Continue Reading

Citations

Feb 9, 2002·Journal of Biomedical Materials Research·Rene S Rosenson-SchlossPrabhas V Moghe
Dec 13, 2017·The Journal of Surgical Research·Robert D KirktonShannon L M Dahl
May 30, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Jonas WetteröKarl-Eric Magnusson

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