Quantitative analysis of topoisomerase II alpha and evaluation of its effects on cell proliferation and apoptosis in glioblastoma cancer stem cells

Neuroscience Letters
Yang HongYun-Hui Liu

Abstract

DNA topoisomerase II alpha (Topo-IIα) is an essential nuclear enzyme with its up-regulation demonstrated in different tumors. This study is to evaluate the expression of Topo-IIα in glioblastoma cancer stem cells (CSCs) and its effects on cell proliferation and apoptosis. We observed the expression differences of Topo-IIα gene transcript in CSCs and non-CSCs in glioblastoma cell line U87 by quantitative real-time PCR and western blot; we silenced Topo-IIα expression in U87 CSCs using Topo-IIα-specific siRNA to investigate the role of Topo-IIα in cell proliferation, cell cycle, and apoptosis. We found higher expression of Topo-IIα in CSCs than in non-CSCs. After using Topo-IIα-specific siRNA to silence the expression of Topo-IIα gene, we observed decreasing cell proliferation, cell cycle arrest, and induction of apoptosis in CSCs, which suggest that Topo-IIα contributes to both cell proliferation and/or cell survival. Topo-IIα may be a novel marker of cell proliferation and/or survival factor in glioblastoma CSCs. It may be a potential target that will open up new opportunities to develop therapeutic strategies for gliomas.

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Citations

Nov 7, 2015·Biochemical and Biophysical Research Communications·Jing LiuYun-Hui Liu
Jul 18, 2015·Chinese Journal of Cancer·Meiqi ZengOu Sha
Oct 21, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yousaf Ali, Shafida Abd Hamid
Feb 13, 2015·European Journal of Nuclear Medicine and Molecular Imaging·Monique R BernsenMarion de Jong
Dec 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingyi SongNingning He

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