Quantitative analysis of urinary glycerol levels for doping control purposes using gas chromatography-mass spectrometry

European Journal of Mass Spectrometry
Mario ThevisWilhelm Schänzer

Abstract

The administration of glycerol to endurance athletes results in an increased fluid retention and improved performance, particularly under hot and humid conditions. Consequently, glycerol is considered relevant for sports drug testing and methods for its detection in urine specimens are required. A major issue in this regard is the natural occurrence of trace amounts of glycerol in human urine, which necessitates a quantitative analysis and the determination of normal urinary glycerol levels under various sporting conditions. A quantitative method was established using a gas chromatography/isotope-dilution mass spectrometry-based approach that was validated with regard to lower limit of detection (0.3 microg mL(-1)), lower limit of quantification (0.9 microg mL(-1)), specificity, linearity (1.0-98.0 microg mL(-1)), intraday and interday precision (<20% at 2.4, 24.1 and 48.2 microg mL(-1)) as well as accuracy (92-110%). Sample aliquots of 20 microL were enriched with five-fold deuterated glycerol, dried and derivatised using N-methyl-trimethylsilyltrifluoroacetamide (MSTFA) before analysis. The established method was applied to a total of 1039 doping control samples covering various sport disciplines (349 endurance samples, 286 s...Continue Reading

References

Jun 1, 1972·The Journal of Clinical Investigation·W M BortzW L Holmes
Mar 1, 1982·Journal of Neurosurgery·S L Wald, R L McLaurin
Jan 1, 1996·International Journal of Sports Medicine·P MontnerT W Chick
Dec 1, 1995·Journal of Applied Physiology·B J FreundC R Valeri
Nov 5, 1998·Sports Medicine·R A Robergs, S E Griffin
Sep 29, 1999·European Journal of Applied Physiology and Occupational Physiology·S HitchinsG P Dobson
Mar 20, 2001·International Journal of Sport Nutrition and Exercise Metabolism·T P ScheettK D Wagoner
Oct 10, 2001·International Journal of Sport Nutrition and Exercise Metabolism·M J AndersonM A Febbraio
May 8, 2002·International Journal of Sport Nutrition and Exercise Metabolism·Aaron CouttsMark Holmes
Jan 25, 2003·Medicine and Science in Sports and Exercise·Meir MagalJoseph C Boyd
Apr 16, 2003·Pflügers Archiv : European journal of physiology·Frank E MarinoJack Cannon
Apr 11, 2006·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Eric D B GouletIsabelle J Dionne
Oct 24, 2007·Sports Medicine·Jeff L Nelson, Robert A Robergs
Oct 27, 2007·International Journal of Sport Nutrition and Exercise Metabolism·Eric D B GouletIsabelle J Dionne

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Citations

Nov 1, 2012·Nutrients·Thelma P PolyviouYannis P Pitsiladis
Jan 22, 2011·Bioanalysis·Ruth E SimoniFrancisco R Aquino Neto
Mar 18, 2016·Forensic Science International : Synergy·Masato OkanoShinji Kageyama
Dec 24, 2015·Drug Testing and Analysis·Koen Deventer, Peter Van Eenoo
Oct 16, 2015·Bioanalysis·Ioanna AthanasiadouYiannis S Angelis
Oct 21, 2011·Drug Testing and Analysis·Karsten KoehlerWilhelm Schaenzer
Dec 20, 2013·Drug Testing and Analysis·Brian N KellyDaniel Eichner
Mar 16, 2013·Drug Testing and Analysis·Brian N KellyDavid E Bruns
Aug 1, 2014·Drug Testing and Analysis·Masato OkanoShinji Kageyama
Jun 27, 2015·Drug Testing and Analysis·Vinícius F SardelaFrancisco R Aquino Neto
Apr 20, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ying DongJinglin Ding
Jun 4, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yixiao Shen, Zhimin Xu
Jan 26, 2012·Clinical Chemistry·Selvin H EdwardsMary M Kimberly

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