PMID: 3744566Jan 1, 1986Paper

Quantitative analysis of urinary glycine conjugates by high performance liquid chromatography: excretion of hippuric acid and methylhippuric acids in the urine of subjects exposed to vapours of toluene and xylenes

International Archives of Occupational and Environmental Health
M Ogata, T Taguchi

Abstract

A new method for the direct determination of hippuric acid (HA) and o-, m- and p-methylhippuric acids (MHAs) in the urine, metabolites of toluene and o-, m- and p-xylenes by high performance liquid chromatography (HPLC) is described. A stainless-steel column packed with silica gel having dinitrophenyl residue and a mixed solution of methanol/water/acetic acid (80/20/0.2) containing tetra-n-butylammonium bromide (0.2% w/v) as mobile phase was used. Concentrations of HA and MHAs were estimated from their peak height at a wave length of 225 nm. Urine can be analyzed directly without solvent extraction or pretreatment to obtain complete separation of HA and o-, m- and p-MHAs. Urine samples from male workers exposed to toluene or xylenes were analyzed for HA or MHAs. The urinary levels of HA and MHAs increased by exposure to toluene and xylenes in proportion to the environmental concentrations of the solvents, although there is a considerable variation in metabolite concentrations. The slope of regression line between toluene and HA and that between m-xylene and m-MHA were similar. The urinary concentrations of HA and MHAs corresponding to 100 ppm (TLV) of toluene was 2.35 g/g creatinine and that of m-MHA corresponding to 100 ppm (T...Continue Reading

References

Jul 14, 1978·International Archives of Occupational and Environmental Health·R Sugihara, M Ogata
Jul 5, 1976·International Archives of Occupational and Environmental Health·V Sedivec, J Flek
Jun 1, 1971·American Industrial Hygiene Association Journal
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Jul 1, 1969·British Journal of Industrial Medicine·M Ikeda, H Ohtsuji
Jan 1, 1983·International Archives of Occupational and Environmental Health·K HasegawaM Ikeda

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Citations

Jul 1, 1995·Archives of Environmental Contamination and Toxicology·M YoshikawaY Kodama
Jan 1, 1986·International Archives of Occupational and Environmental Health·M OgataT Kawai
Sep 1, 1994·Archives of Environmental Health·T KawamotoS Matsuda
Dec 1, 1994·Occupational and Environmental Medicine·T KawamotoY Kodama
Jan 1, 1991·International Archives of Occupational and Environmental Health·D de CarvalhoS A Dreossi
May 8, 2016·International Journal of Biological Macromolecules·Ozden Canli TasarMesut Taskin
May 6, 2011·Toxicology and Industrial Health·Hsu-Tzu ShihHisen-Wen Kuo
May 24, 2006·Industrial Health·Fumiyuki AsakawaFumihiko Jitsunari
Jul 1, 2011·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Hueiwang Anna JengMing-Tsang Wu
Dec 17, 2008·International Journal of Occupational Safety and Ergonomics : JOSE·Hamid-Reza HeidariAbbas Rahimi-Froushani
Sep 16, 1992·Journal of Chromatography·J Angerer, B Hörsch
May 8, 2021·International Journal of Hygiene and Environmental Health·Denise S TevisVíctor R De Jesús

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