Quantitative analysis of urinary phospholipids found in patients with breast cancer by nanoflow liquid chromatography-tandem mass spectrometry: II. Negative ion mode analysis of four phospholipid classes

Analytical and Bioanalytical Chemistry
Hye Kyeong MinMyeong Hee Moon

Abstract

Analysis was performed on four different categories of phospholipids (phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidic acid (PA)) from urine in patients with breast cancer. This quantitative analysis was conducted using nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nLC-ESI-MS-MS). This study shows the profiling of the phospholipids (PLs) that can be identified by the negative ion mode of MS. A previous study (Kim et al. Anal. Bioanal. Chem. 393:1649, 21) focused on only two PL classes: phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs) and were identified by positive ion mode. PLs were extracted by lyophilization of 1 mL of urine from both healthy normal females and breast cancer patients before and after surgery. Separation of PLs was performed by nLC followed by structural identification of PLs using data-dependent collision-induced dissociation. A total of 34 urinary PL molecules (12 PSs, 12 PIs, four PGs, and six PAs) were quantitatively examined. Among the four PL categories examined in this study, most PL classes showed an increase in the total amounts in the cancer patients, yet PIs exhibited some decreases. The present study su...Continue Reading

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Citations

Aug 29, 2013·Lipids·Francesca GiuffridaMarcel Braun
Aug 1, 2012·Journal of the American Society for Mass Spectrometry·Kentaro YoshimuraSen Takeda
Jan 15, 2016·Biomedical Chromatography : BMC·Sumitra PatiBrian S Cummings
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Oct 16, 2012·Analytical and Bioanalytical Chemistry·Corinne BuréJean-Marie Schmitter
Oct 19, 2010·Analytical and Bioanalytical Chemistry·Hye Kyeong MinMyeong Hee Moon
Sep 25, 2020·BMC Medical Informatics and Decision Making·Xiaoli ChenYouping Deng
Oct 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui GuoYouping Deng
Jan 10, 2013·Journal of Agricultural and Food Chemistry·Bertram FongCarmen Norris

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