Quantitative analysis of UV photolesions suggests that cyclobutane pyrimidine dimers produced in mouse skin by UVB are more mutagenic than those produced by UVC

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Hironobu IkehataMasayuki Yamamoto

Abstract

The amount of photolesions produced in DNA after exposure to physiological doses of ultraviolet radiation (UVR) can be estimated with high sensitivity and at low cost through an immunological assay, ELISA, which, however, provides only a relative estimate that cannot be used for comparisons between different photolesions such as cyclobutane pyrimidine dimer (CPD) and pyrimidine(6-4)pyrimidone photoproduct (64PP) or for analysis of the genotoxicity of photolesions on a molecular basis. To solve this drawback of ELISA, we introduced a set of UVR-exposed, calibration DNA whose photolesion amounts were predetermined and estimated the absolute molecular amounts of CPDs and 64PPs produced in mouse skin exposed to UVC and UVB. We confirmed previously reported observations that UVC induced more photolesions in the skin than UVB at the same dose, and that both types of UVR produced more CPDs than 64PPs. The UVR protection abilities of the cornified and epidermal layers for the lower tissues were also evaluated quantitatively. We noticed that the values of absorbance obtained in ELISA were not always proportional to the molecular amounts of the lesion, especially for CPD, cautioning against the direct use of ELISA absorbance data for est...Continue Reading

References

Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D E BrashJ Pontén
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A GossenJ Vijg
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R B Setlow
May 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R B SetlowF J Bollum
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D C Thomas, T A Kunkel
Jan 1, 1993·Molecular and Cellular Biology·M P CartyK Dixon
Nov 1, 1995·Japanese Journal of Cancer Research : Gann·X QinT Ishikawa
Oct 16, 2002·Mutation Research·Hironobu Ikehata, Tetsuya Ono
Jan 15, 2003·The Journal of Biological Chemistry·Dong-Hyun Lee, Gerd P Pfeifer
Apr 30, 1963·Biochimica Et Biophysica Acta·R A DEERING, R B SETLOW
Dec 22, 2004·Mechanisms of Ageing and Development·T von ZglinickiS P Jackson
Jan 26, 2005·Current Biology : CB·Judith JansGijsbertus T J van der Horst
Mar 8, 2005·Mutation Research·Jean CadetThierry Douki
Sep 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stéphane MouretThierry Douki
Feb 28, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·André Passaglia SchuchCarlos Frederico Martins Menck
Dec 17, 2009·Photochemistry and Photobiology·David Mitchell, Becky Brooks
Mar 26, 2011·Journal of Radiation Research·Hironobu Ikehata, Tetsuya Ono
Oct 19, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thomas M RüngerAdam Rees
Apr 11, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thierry Douki
Jul 24, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Nathalie BastienRégen Drouin
Nov 6, 2014·Topics in Current Chemistry·Jean CadetThierry Douki
Oct 8, 2016·Photochemistry and Photobiology·Peng MaoMichael J Smerdon

❮ Previous
Next ❯

Citations

Aug 14, 2018·Physical Chemistry Chemical Physics : PCCP·Akos BanyaszDimitra Markovitsi
Aug 29, 2019·Photochemistry and Photobiology·Hironobu IkehataMasayuki Yamamoto
Mar 3, 2020·Photochemistry and Photobiology·Jihoon W LeeMasaoki Kawasumi
Jul 26, 2019·Cellular and Molecular Life Sciences : CMLS·Karolina BoguszewskaBolesław T Karwowski
Feb 11, 2021·International Journal of Molecular Sciences·Eszter FidrusÉva Remenyik
Jun 17, 2021·Photochemistry and Photobiology·Maurício S BaptistaAndrés H Thomas

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
transgenic

Software Mentioned

JMP Pro
KaleidaGraph

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.