Quantitative and qualitative approach of glycan-glycan interactions in marine sponges

Biochimie
O PopescuG N Misevic

Abstract

Cell recognition and adhesion involving many kinds of cell surface molecules operate via homotypic and/or heterotypic protein-protein and protein-carbohydrate binding. Our investigations in marine sponges have provided direct evidence for a novel molecular mechanism of multivalent glycan-glycan binding related to cellular interactions. Biochemical characterization of purified proteoglycans revealed the presence of specific acidic glycans, different from classical glycosaminoglycans. Such acidic glycans of high molecular weight, containing fucose, glucuronic or galacturonic acids, and pyruvate and sulfate groups may represent a new class of primordial proteoglycans, named by us glyconectins. The thermodynamic and kinetic approaches of biological macromolecule interactions do not provide a direct measurement of the intermolecular binding forces that are fundamental for the function of the ligand-receptor association. Using the atomic force microscopy (AFM), we provided the first quantitative evaluation of the binding strength between cell adhesion proteoglycans. Measurement of binding forces intrinsic to cell adhesion glyconectin proteoglycans (AGPs) is necessary to assess their contribution to the maintenance of the anatomical i...Continue Reading

References

Jan 1, 1995·Journal of Molecular Recognition : JMR·G N Misevic, O Popescu
Oct 1, 1993·Journal of Cellular Biochemistry·E Papakonstantinou, G N Misevic
Jan 18, 1996·Nature·J Israelachvili, H Wennerström
Mar 20, 1997·Nature·O Popescu, G N Misevic
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·S R HaseleyJ F Vliegenthart

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Citations

Dec 18, 2015·Proceedings of the National Academy of Sciences of the United States of America·Christopher J DayMichael P Jennings
Aug 5, 2008·Biophysical Journal·Lin HanChristine Ortiz
Oct 4, 2006·Micron : the International Research and Review Journal for Microscopy·Chih-Kung LeeShiming Lin
Oct 25, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Rita LangascoElisabetta Gavini
Apr 21, 2018·Nature Reviews. Microbiology·Jessica PooleMichael P Jennings
Dec 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Fenja KnoeppMartin Fronius
Aug 19, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Hashanthi K Abeyratne-Perera, Preethi L Chandran

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