Quantitative and qualitative characteristics of frozen-thawed bovine spermatozoa recovered via a conventional and a standardized swim-up technique

The Tohoku Journal of Experimental Medicine
J R CorreaP M Zavos

Abstract

The objective of this study was to use the bovine as a model to evaluate the recovery of frozen-thawed spermatozoa via a conventional and a standardized swim-up technique. Frozen-thawed semen specimens (3 bulls) were washed and reconstituted with 2.9% (w/v) sodium citrate extender containing 20% (v/v) chicken egg yolk (SC-EY). Reconstituted sperm specimens were used for selection via conventional swim-up and the standardized ZSC method. The swim-up method consisted of overlayering the sperm specimen with 0.7 to 1.0 ml of isolation media (Ham's F-10), followed by 1 hr of incubation. The ZSC consisted of a conical cavity on the bottom of a glass column. The sperm specimen was placed into the conical cavity until the surface of the specimen was at the same level as the upper boundaries of the conical cavity. The surrounding periconical and epiconical areas were filled with 0.7 to 1.0 ml of isolation media followed by 1 hr of incubation. The isolation media was removed (harvesting) from swim-up (80% volume) and ZSC specimens (100% volume) at the end of incubation. Recovered specimens were assessed for volume (ml), sperm concentration (x10(6) spermatozoa/ml), the percentage and grade of motility (0 to 4), the occurrence of osmotic s...Continue Reading

References

Jun 1, 1976·Fertility and Sterility·A LopataB James
Jan 1, 1988·Annals of the New York Academy of Sciences·R P MarrsP Quinn
Feb 1, 1984·International Journal of Andrology·A MaklerF Naftolin

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