Quantitative and qualitative immunohistochemistry of T cell subsets and MHC class II expression in the canine small intestine

Veterinary Immunology and Immunopathology
C M ElwoodR M Batt

Abstract

In man and rodents, cells of the gastrointestinal immune system include B and T lymphocytes, granulocytes, macrophages and dendritic cells. Abnormalities in leucocyte numbers and function have been described in diseases of humans, such as coeliac disease and inflammatory bowel disease. The purpose of this study is to describe the normal distribution of T cells and MHC Class II expression in the small intestine of clinically normal dogs, to allow subsequent comparison with disease states. Full-thickness sections of duodenum, jejunum and ileum from seven young adult beagle dogs were immediately snap-frozen following euthanasia. Avidin-biotin-enhanced immunocytochemistry was used to detect expression of canine CD3, CD4, CD8 and MHC Class II antigens. Positively stained lamina propria cells were quantified using an eyepiece graticule, and positively stained intraepithelial cells by counts per 100 epithelial cells. In the lamina propria, the density of all leucocyte subsets was significantly increased towards the villus tip for all regions (p < 0.05). There was no apparent difference in the distribution of CD3+, CD4+ and CD8+ leucocytes between the three portions of the small intestine. The ratios of CD4+ cells to CD8+ cells in the ...Continue Reading

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Apr 15, 2010·Journal of Veterinary Internal Medicine·R J WashabauUNKNOWN WSAVA International Gastrointestinal Standardization Group
Mar 24, 2012·Veterinary Research·Johannes JungingerMarion Hewicker-Trautwein
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Apr 14, 2006·Veterinary Research·Chris Stokes, Nashwa Waly
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Jun 1, 2013·Innate Immunity·Johannes JungingerMarion Hewicker-Trautwein
Jun 1, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·David K Meyerholz, Amanda P Beck

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