Quantitative angiogenesis in a syngeneic tumor spheroid model

Microvascular Research
I P Torres FilhoP Borgström

Abstract

The aim of this work was to develop a system for noninvasive, in vivo, and in situ study of tumor angiogenesis in awake mice. Tumor spheroids were prepared from Lewis lung carcinoma cells prelabeled with methylrhodamine. A transparent chamber consisting of two titanium frames was implanted into the dorsal skin of CB6 mice. One layer of the skin was removed in a 15-mm area and covered with a coverslip. A few days later, the coverslip was removed and one to three tumor spheroids (diameters, 500-900 microns) were placed over the upper tissue layer. The selected fields were recorded under trans- and epi-illumination using video microscopy. Separate fluorescence filter sets were used to visualize the FITC-labeled plasma and the rhodamine-labeled tumor spheroids. The dual labeling technique allowed precise identification of the tumors and the study of tumor and microvessel growth for up to 14 days. The tumor area and morphometric parameters of tumor vessels were measured from recorded images. After implantation, tumor cells formed well-defined tumor foci. Venular and capillary dilation and tortuosity were observed in the surrounding tissue 1-2 days after implantation, followed by the appearance of buds and sprouts. After that, vascul...Continue Reading

Citations

Jan 13, 2006·British Journal of Cancer·L BingleN J Brown
Dec 1, 2006·Expert Opinion on Drug Discovery·Divya Khaitan, B S Dwarakanath
Feb 19, 2000·Circulation Research·V DjonovP H Burri
Jun 27, 2019·Stem Cell Research & Therapy·Tahir MuhammadYinghui Huang
Oct 18, 2000·Critical Reviews in Oncology/hematology·W Mueller-Klieser

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