Quantitative approaches to detect donor and passage differences in adipogenic potential and clonogenicity in human bone marrow-derived mesenchymal stem cells.

Tissue Engineering. Part C, Methods
Jessica Lo Surdo, Steven R Bauer

Abstract

Bone marrow-derived multipotent stromal cells (MSCs), also known as mesenchymal stem cells, have great promise due to their capacity for tri-lineage differentiation and immunosuppressive properties, which allows for their allogeneic use and ultimately may allow for treatment of many diseases. MSCs will require extensive expansion and passaging to obtain cells in sufficient numbers necessary for cell therapies. MSCs from many donors could potentially be used. Because of this, there is a need to understand the role of passaging and donor differences on differentiation capacity using quantitative approaches. Here, we evaluated MSCs from two donors (noted as PCBM1632 and PCBM1641 by the manufacturer) at tissue culture passages 3, 5, and 7. We used a colony forming unit (CFU) assay and limiting dilution to quantify clonogenicity and precursor frequency during adipogenesis, and quantitative real-time-polymerase chain reaction for adipogenic markers to evaluate changes on a gene expression level. Further, we observed changes in cell size, and we sorted small and large populations to evaluate size-related adipogenic potential. While the adipogenic precursor frequency of ∼1 in 76 cells remained similar through passages for cells from PC...Continue Reading

References

Feb 26, 1987·Journal of Immunological Methods·L W StrijboschG Groenewegen
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·D C ColterD J Prockop
Aug 10, 2000·Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft für Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft für Mikrochirurgie der Peripheren Nerven und Gefässe : Organ der Vereinigung der Deut·R Koller, M Frey
May 19, 2001·Methods in Molecular Biology·T FrisanM Masucci
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·D C ColterD J Prockop
Aug 3, 2001·Blood Cells, Molecules & Diseases·M W Long
Aug 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A A WorsterA J Nixon
Jan 11, 2002·Blood Cells, Molecules & Diseases·B M DeasyJ Huard
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 29, 2002·Gene Therapy·R J JankowskiJ Huard
Jan 17, 2003·Obesity Research·Lenka JanderováSteven R Smith
Mar 21, 2003·Biochemical and Biophysical Research Communications·Takanori NakamuraGozoh Tsujimoto
Oct 11, 2003·Experimental Hematology·Katarina Le BlancOlle Ringdén
Feb 19, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ichiro SekiyaDarwin J Prockop
Sep 24, 2004·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Dong-Feng ChenMin Xu
Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
Nov 24, 2004·Gene·Shih-Chieh HungLarry Low-Tone Ho
Jan 18, 2005·Cell and Tissue Research·Darko BosnakovskiToru Fujinaga
Jun 1, 2005·Experimental Cell Research·Carl A GregoryJeffrey L Spees
Oct 12, 2005·Plastic and Reconstructive Surgery·Lauren E KokaiKacey G Marra
Oct 3, 2006·European Journal of Immunology·Antonio UccelliVito Pistoia
Jun 29, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Agnieszka BanasTakahiro Ochiya
Nov 23, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jeffrey L SpeesDarwin J Prockop
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·Laura de GirolamoAnna T Brini
Dec 13, 2007·Stem Cell Reviews·Wolfgang Wagner, Anthony D Ho
Dec 19, 2007·Methods in Molecular Medicine·Bruno Delorme, Pierre Charbord
Mar 29, 2008·Methods in Molecular Biology·Margaret WolfeRoxanne L Reger
Dec 3, 2008·Methods in Molecular Biology·Jorge CarneiroElisabetta Padovan
Dec 20, 2008·Journal of Biomedical Materials Research. Part a·Tonye BriggsTreena Livingston Arinzeh
Dec 25, 2008·Regenerative Medicine·Mario Cherubino, Kacey G Marra
Jan 16, 2009·International Journal of Laboratory Hematology·A NasefA Chapel
Feb 24, 2009·Journal of Biomedical Materials Research. Part a·Hye-Joung KimGun-Il Im
May 14, 2009·Transplantation·Georg SiegelFrancesco Dazzi
Aug 19, 2009·Stem Cells and Development·Byung Sun YoonSeungkwon You

❮ Previous
Next ❯

Citations

Jan 17, 2013·Stem Cells and Development·Micaela Johanna Glat, Daniel Offen
May 2, 2014·Stem Cell Research & Therapy·Ian H BellayrRaj K Puri
Apr 23, 2014·Regenerative Medicine·Kim L M GardienMagda M W Ulrich
Oct 31, 2013·Tissue Engineering. Part B, Reviews·Xiang-Zhen YanFang Yang
Oct 2, 2013·BioResearch Open Access·Elizabeth Rosado BalmayorReinhold G Erben
Jan 20, 2016·Cytotherapy·Brian G StultzDeborah A Hursh
Jan 23, 2016·Tissue Engineering. Part C, Methods·Kimberly M FerlinJohn P Fisher
May 23, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Rachael AnatolIssam Zineh
Dec 15, 2015·Scientific Reports·Sarah SundelacruzDavid L Kaplan
Nov 27, 2015·Science Translational Medicine·Russ B AltmanKathleen M Giacomini
Apr 3, 2016·Stem Cells and Development·Ian H BellayrRaj K Puri
Jan 7, 2015·Nature Reviews. Endocrinology·Warren L GraysonJeffrey M Gimble
Jun 13, 2017·Radiation Research·Andrea L DiCarloJean-Rene Jourdain
Jan 23, 2018·Biopreservation and Biobanking·Sepideh Jalilzadeh-TabriziNosratollah Zarghami
May 29, 2014·Plastic and Reconstructive Surgery·Benjamin LeviPaul S Cederna
Jan 4, 2020·Journal of Cellular Biochemistry·Monika GasiūnienėRūta Navakauskienė
Nov 30, 2016·Nutrition & Metabolism·Ines WarnkeJoseph Schwager
May 29, 2018·Scientific Reports·Shahensha ShaikRam Devireddy
Apr 20, 2018·Annals of Biomedical Engineering·Nicholas R LabriolaEric M Darling

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
PCR
flow cytometry

Software Mentioned

Excel
FlowJo Analysis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.