PMID: 6160132Jan 1, 1980Paper

Quantitative aspects of the cytochemical Feulgen-DNA procedure studied on model systems and cell nuclei

Histochemistry
A C Van Prooijen-KnegtM van der Ploeg

Abstract

Quantitative aspects of DNA losses during fixation and pararosaniline(SO2)-Feulgen staining of microscopic preparations were studied. The preparation of a new cytochemical model, consisting of DNA-protein layers (with thicknesses between 0.1 and 5.0 micrometer) on microscopic glass slides is described and potentialities and limitations of this model are discussed. Polyacrylamide films into which high molecular weight calf thymus DNA or chicken erythrocyte nuclei had been constrained served as another model. As biological objects chicken erythrocyte nuclei and rat liver nuclei either in suspension or on microscopical glass slides were used. The experimental results indicate a loss of about 5% of the DNA due to the fixation procedure applied. Hydrolysis in 5 N HCl at room temperature, staining with the pararosaniline-Schiff medium and rinsing with sulfurous acid induced losses of DNA too, varying in amount depending on the type of preparation used. About 10% of the original DNA content is lost in total from chicken erythrocyte nuclei and rat liver nuclei dried on microscopical glass slides, from chicken erythrocyte nuclei constrained in polyacrylamide films, and from DNA-protein layers on microscopic glass slides. For nuclei fixe...Continue Reading

References

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Citations

Apr 1, 1985·The Histochemical Journal·D Hopwood
Jul 1, 1982·Molecular and Biochemical Parasitology·P BorstJ James
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F Zampetti-BosselerM Steinert
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R F KooyJ P Overdulve
Jun 2, 2016·Nature Plants·George O Poinar, Lena Struwe
Jan 1, 1986·Histochemistry·M van der Ploeg, W A Duijndam

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