PMID: 6160130Nov 1, 1980Paper

Quantitative aspects of the cytochemical demonstration of glucose-6-phosphate dehydrogenase with tetranitro BT studied in a model system of polyacrylamide films

The Histochemical Journal
C J Van Noorden, J Tas

Abstract

The cytochemical determination of the activity of glucose-6-phosphate dehydrogenase (G6PDH) with tetranitro blue tetrazolium (TNBT) was studied with model films of polyacrylamide gel incorporating purified enzyme. This model system enabled a quantitative study to be made of different parameters involved with the cytochemical assay as it is applied to sections or smears. The enzyme activity of G6PDH incorporated in the model films was also assayed biochemically. Optimal conditions for retaining the maximum amount of enzymic activity are described. The behaviour of G6PDH towards enzyme inhibitors was found to be similar in model films and in solution. WIth TNBT, absorbance measurements at a single wavelength (535 nm) were used to estimate the enzyme activity quantitatively. When carried out under standardized conditions, both the cytochemical and biochemical assay showed a linear relation with the time of incubation and obeyed the Beer-Lambert law. The correlation between biochemical and cytochemical data was very high, which enabled cytochemical data to be converted into absolute units of enzyme activity. The data obtained in this way closely resembled the data of enzyme activity calculated from the absorbance of formazan produc...Continue Reading

References

Nov 1, 1976·The Histochemical Journal·P van Duijn
May 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A S De JongW T Daems
Mar 10, 1977·The New England Journal of Medicine·G Weber
Feb 15, 1979·Cell and Tissue Research·W HosemannD Sasse
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Mar 1, 1976·The Histochemical Journal·T H Rosenquist
Aug 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P Van DuijnM Van der Ploeg
Sep 1, 1973·The Histochemical Journal·J Tas, R C Roozemond
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Nov 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M van der Ploeg, P van Duijn
Jan 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C J Van Noorden, J Tas

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Citations

Sep 1, 1982·The Histochemical Journal·C J Van Noorden, J Tas
Jun 1, 1983·The Histochemical Journal·C J Van NoordenI M Vogels
Jan 1, 1985·Archives of Andrology·A Abou-HailaM A Fain-Maurel
May 5, 2016·Progress in Histochemistry and Cytochemistry·Adriano AzaripourCornelis J F Van Noorden
Jan 1, 1986·Histochemistry·M van der Ploeg, W A Duijndam

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