Quantitative assay for lectin-induced cytoagglutination by means of an electronic particle-counting technique

Journal of the National Cancer Institute
G P PhondkeR F Barth

Abstract

A method employing an electronic particle-counting technique was used to quantify lectin-induced agglutination of human granulocytes and lymphocytes with either concanavalin A or wheat germ agglutinin. The number and mean volume of single cells and aggregates in the presence of increasing concentrations of lectin were computed from 95% confidence intervals. Agglutination depended on both the number of free cells and the number and size of the cell clusters. Changes in these two variables were mutually independent of one another, and both were simultaneously determined. An index of agglutination that takes the effect of these two variables into account was defined as (formula: see text) VA equals mean volume of cell aggregates, NS equals number of single cells, NA equals number of cell aggregates, VS equals mean volume of single cells, rb equals r at a given lectin concentration, and ra equals r in the absence of lectin. For any combination of lectin and cell type, the agglutination curve, as described by zeta, consisted of two components: a) a flat region in which zeta remained constant with increasing lectin concentrations and b) a region in which zeta increased linearly as a function of the logarithm of lectin concentration. ...Continue Reading

Citations

Nov 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G Z TauP B Rothman
Jan 1, 1982·Cancer Metastasis Reviews·G Poste
Feb 1, 1990·Cancer Metastasis Reviews·P N Belloni, R J Tressler

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