Quantitative assessment of antibodies to ribonucleoproteins in primary Sjögren syndrome: correlation with B-cell biomarkers and disease activity

Annals of the Rheumatic Diseases
Sophie CandonX Mariette

Abstract

To assess the added value of using a radioligand assay (RLA) compared with ELISA to detect antibodies to SSA, SSB and RNP, and to analyse the correlation between autoantibody levels, B-cell biomarkers and disease activity. Antibodies to SSA, SSB and RNP were assessed in 127 patients with primary Sjögren syndrome (pSS) using an RLA and ELISA. In parallel, measures of B-cell activation were determined including serum levels of B-cell-activating factor of the tumour necrosis factor family or BLyS (BAFF). RLA was more sensitive than ELISA for the detection of antibodies to SSB (54% of positive samples versus 37%, respectively) and antibodies to RNP (9% vs 3%). No difference was seen for the sensitivity of detection of antibodies to SSA. Anti-SSA and anti-SSB levels were correlated with both techniques. Mean levels of antibodies to SSA were significantly higher in patients presenting antibodies to both SSA and SSB than in those exhibiting antibodies to SSA only (RLA: mean (SEM) anti-SSA levels 2343 (158) cpm vs 1348 (286) cpm, respectively, p = 0.02; ELISA: 6.8 (0.8) vs 3.8 (0.4), respectively, p = 0.003). Levels of antibodies to SSA and SSB significantly correlated with those of circulating BAFF (r = 0.4, p = 0.004 and r = 0.6, p<0...Continue Reading

Citations

Jul 17, 2013·Nature Reviews. Rheumatology·Gaëtane Nocturne, Xavier Mariette
Feb 22, 2012·Therapeutics and Clinical Risk Management·Laurent ChicheJulien Mancini
Mar 24, 2010·Rheumatology International·Sule YavuzEbru Toker
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Feb 28, 2014·Annals of the Rheumatic Diseases·Xiang LinLiwei Lu
Jul 18, 2019·Clinical and Experimental Immunology·C XieY Fu
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May 6, 2015·International Journal of Rheumatic Diseases·Fengyuan DengZuguo Liu

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