PMID: 9186945May 1, 1997Paper

Quantitative assessment of hepatitis C virus RNA by polymerase chain reaction and a digoxigenin detection system: comparison with branched DNA assay

Journal of Virological Methods
C T YehY F Liaw

Abstract

A method for quantifying hepatitis C virus (HCV) RNA in serum using reverse transcription-polymerase chain reaction (RT-PCR) followed by slot-blot hybridization with a specific, digoxigenin-labeled probe was developed. Using RNA synthesized from cloned HCV cDNA as a standard, serum concentration of HCV RNA above 10 copies/ml can be quantitatively determined. To compare this method with branched DNA (bDNA) assay, 45 serum samples from 26 patients with newly acquired acute hepatitis C (n = 16) or hepatitis C with acute exacerbation (n = 10) were submitted to both assays. HCV RNA in 30 (67%), 12 (27%) and three (6.7%) samples can be quantitatively determined by both, either and none of the two assays, respectively. Using a standardized qualitative HCV RNA detection test (Amplicor HCV test) as a reference, 1 and 0 false positive results were found by bDNA and this assay, respectively. This quantitative assay using RT-PCR and a digoxigenin detection system was comparable to bDNA assay. Since a false positive result was rarely found, this technique can be used as a first line test to screen a large number of samples rapidly and economically.

References

Aug 1, 1992·Journal of Medical Virology·S KanekoK Kobayashi
Jul 11, 1991·The New England Journal of Medicine·P FarciR H Purcell
Jun 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K ChayamaH Kumada
Apr 21, 1990·Lancet·S KanekoN Hattori
Apr 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y F LiawC C Pao
Nov 30, 1989·The New England Journal of Medicine·A M Di BisceglieJ H Hoofnagle
May 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C M ChuH C Thomas

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Citations

Oct 3, 2003·Biochemical and Biophysical Research Communications·Chau-Ting YehJohn T A Hsu
Feb 12, 2009·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Ming-Wei LaiChau-Ting Yeh
May 14, 2008·Biochemical and Biophysical Research Communications·Chau-Ting YehJu-Chien Cheng
Mar 24, 2005·World Journal of Gastroenterology : WJG·Cho-Li YenLiang-Che Chang

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