Quantitative assessment of passive electrical properties of the cardiac T-tubular system by FRAP microscopy

Proceedings of the National Academy of Sciences of the United States of America
M ScardigliLeonardo Sacconi

Abstract

Well-coordinated activation of all cardiomyocytes must occur on every heartbeat. At the cell level, a complex network of sarcolemmal invaginations, called the transverse-axial tubular system (TATS), propagates membrane potential changes to the cell core, ensuring synchronous and uniform excitation-contraction coupling. Although myocardial conduction of excitation has been widely described, the electrical properties of the TATS remain mostly unknown. Here, we exploit the formal analogy between diffusion and electrical conductivity to link the latter with the diffusional properties of TATS. Fluorescence recovery after photobleaching (FRAP) microscopy is used to probe the diffusion properties of TATS in isolated rat cardiomyocytes: A fluorescent dextran inside TATS lumen is photobleached, and signal recovery by diffusion of unbleached dextran from the extracellular space is monitored. We designed a mathematical model to correlate the time constant of fluorescence recovery with the apparent diffusion coefficient of the fluorescent molecules. Then, apparent diffusion is linked to electrical conductivity and used to evaluate the efficiency of the passive spread of membrane depolarization along TATS. The method is first validated in c...Continue Reading

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Citations

Jan 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·M ScardigliL Sacconi
Jan 8, 2018·Proceedings of the National Academy of Sciences of the United States of America·Emilia Entcheva
Jul 11, 2018·The Journal of Physiology·M ScardigliL Sacconi
Nov 27, 2018·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Eva Alicja Rog-ZielinskaPeter Kohl
Mar 30, 2019·The Journal of Physiology·C CrociniL Sacconi
Jan 11, 2020·Frontiers in Physiology·Sarah Helena VermijJan Pavel Kucera
Oct 24, 2018·Frontiers in Physiology·Sören BrandenburgStephan E Lehnart
Oct 3, 2019·Physical Review. E·Alessandro LoppiniSimonetta Filippi
Jul 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Cherrie H T KongMark B Cannell
Nov 10, 2018·Frontiers in Physiology·Marina ScardigliLeonardo Sacconi
Nov 23, 2020·Journal of Muscle Research and Cell Motility·Giulia VitaleCecilia Ferrantini
Nov 25, 2020·Circulation Research·Eva A Rog-ZielinskaPeter Kohl
May 28, 2019·Progress in Biophysics and Molecular Biology·Claudia CrociniLeslie A Leinwand
Jul 4, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·S H VermijJ P Kucera

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