Quantitative assessment of peptide-lipid interactions. Ubiquitous fluorescence methodologies

Biochimica Et Biophysica Acta
Pedro M MatosNuno C Santos

Abstract

Peptide-membrane interactions have been gaining increased relevance, mainly in biomedical investigation, as the potential of the natural, nature-based and synthetic peptides as new drugs or drug candidates also expands. These peptides must face the cell membrane when they interfere with or participate in intracellular processes. Additionally, several peptide drugs and drug leads actions occur at the membrane level (e.g., antimicrobial peptides, cell-penetrating peptides and enveloped viruses membrane fusion inhibitors). Here we explore fluorescence spectroscopy methods that can be used to monitor such interactions. Two main approaches are considered, centered either on the peptide or on the membrane. On the first, we consider mainly the methodologies based on the intrinsic fluorescence of the aminoacid residues tryptophan and tyrosine. Regarding membrane-centric approaches, we review methods based on lipophilic probes sensitive to membrane potentials. The use of fluorescence constitutes a simple and sensitive method to measure these events. Unraveling the molecular mechanisms that govern these interactions can unlock the key to understand specific biological processes involving natural peptides or to optimize the action of a pe...Continue Reading

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Citations

Jan 14, 2011·European Biophysics Journal : EBJ·João M FreireMiguel A R B Castanho
Apr 6, 2011·PloS One·Filomena A CarvalhoNuno C Santos
Jan 17, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·João Miguel FreireMiguel A R B Castanho
Mar 23, 2011·FEBS Letters·Helene J BustadAurora Martinez
Jul 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcelo T AugustoNuno C Santos
Nov 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bárbara GomesMatteo Porotto
May 11, 2019·Soft Matter·Sarah R DennisonDavid A Phoenix
Nov 30, 2012·Biopolymers·Sónia GonçalvesNuno C Santos
Mar 8, 2017·International Journal of Molecular Sciences·Ching-Chia TangLong-Sen Chang
Dec 4, 2020·International Journal of Molecular Sciences·Marcin MakowskiSónia Gonçalves
Nov 23, 2018·Biochimica Et Biophysica Acta. Biomembranes·Luz N IrazazabalOctavio L Franco
Jan 24, 2020·Biophysical Journal·Anja StulzHeiko Heerklotz
Apr 6, 2018·Biochimica Et Biophysica Acta. Biomembranes·Bashiyar AlmarwaniAnderson Sunda-Meya
Mar 3, 2021·Biochimica Et Biophysica Acta. Biomembranes·Anike P V Ferreyra MaillardAxel Hollmann
Apr 28, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Tatiana M DominguesKarin A Riske
Mar 31, 2019·Biochimica Et Biophysica Acta. Biomembranes·Isabel C M FensterseiferOctavio L Franco
Mar 27, 2021·Biochimica Et Biophysica Acta. Biomembranes·André HädickeChristian Salesse
Nov 26, 2013·Bioconjugate Chemistry·Viktoriia Y PostupalenkoYves Mely
Sep 4, 2021·Biochimica Et Biophysica Acta. Biomembranes·Patrícia M SilvaNuno C Santos
Jul 28, 2020·Biochimica Et Biophysica Acta. Biomembranes·Luis Benito Pérez Socas, Ernesto Esteban Ambroggio

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