Quantitative Assessment of Sialo-Glycoproteins and N-Glycans during Cardiomyogenic Differentiation of Human Induced Pluripotent Stem Cells

Chembiochem : a European Journal of Chemical Biology
Sarah A KonzeFalk F R Buettner

Abstract

Human induced pluripotent stem-cell-derived cardiomyocytes (hiPSC CMs) may be used in regenerative medicine for individualized tissue transplants in the future. For application in patients, the generated CMs have to be highly pure and well characterized. In order to overcome the prevalent scarcity of CM-specific markers, we quantitatively assessed cell-surface-exposed sialo-glycoproteins and N-glycans of hiPSCs, CM progenitors, and CMs. Applying a combination of metabolic labeling and specific sialo-glycoprotein capture, we could highly enrich and quantify membrane proteins during cardiomyogenic differentiation. Among them we identified a number of novel, putative biomarkers for hiPSC CMs. Analysis of the N-glycome by capillary gel electrophoresis revealed three novel structures comprising β1,3-linked galactose, α2,6-linked sialic acid and complex fucosylation; these were highly specific for hiPSCs. Bisecting GlcNAc structures strongly increased during differentiation, and we propose that they are characteristic of early, immature CMs.

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Citations

Sep 29, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thomas FischöderLothar Elling
Aug 27, 2019·Frontiers in Cell and Developmental Biology·Amanda LeitolisMarco A Stimamiglio
Mar 3, 2020·Frontiers in Cell and Developmental Biology·Anny Waloski RobertMarco Augusto Stimamiglio
Feb 22, 2021·The Journal of Biological Chemistry·Sina Ibne NoorSabine Strahl
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Mar 13, 2018·Chemical Reviews·Grace LuLisa A Holland
Oct 7, 2021·Electrophoresis·Cristian D Gutierrez-ReyesYehia Mechref

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