Quantitative assessment of telomerase components in cancer cell lines

FEBS Letters
Semih Can AkıncılarVinay Tergaonkar

Abstract

Besides its canonical function of catalyzing the formation of telomeric repeats, many groups have recently reported non-canonical functions of hTERT in particular, and telomerase in general. Regulating transcription is the central basis of non-canonical functions of telomerase. However, unlike reverse transcriptase activity of telomerase that requires only a few molecules of enzymatically active hTERT, non-canonical functions of hTERT or other telomerase components theoretically require several hundred copies. Here, we provide the first direct quantification of all the telomerase components in human cancer cell lines. We demonstrate that telomerase components do not exist in a 1:1 stoichiometric ratio, and there are several hundred copies of hTERT in cells. This provides the molecular basis of hTERT to function in other signaling cascades, including transcription.

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Citations

Feb 6, 2016·Cellular and Molecular Life Sciences : CMLS·Semih Can AkincilarVinay Tergaonkar
May 11, 2015·Nucleic Acids Research·Guillaume KellermannSophie Bombard
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Nov 4, 2016·Cancer Discovery·Semih Can AkıncılarVinay Tergaonkar
Jan 8, 2021·Journal of Gastrointestinal Cancer·Sogand Vahidi, Ali Akbar Samadani
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Feb 19, 2021·Cellular and Molecular Life Sciences : CMLS·Semih Can AkincilarVinay Tergaonkar
May 1, 2021·Cancers·Mathushan SubasriDean H Betts

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