Quantitative assessment of the response of osteoblast- and macrophage-like cells to particles of Ni-free Fe-base alloys

Biomaterials
G CiapettiN Baldini

Abstract

In the present study, the effect of mechanically alloyed particles of new FeAlCr alloys developed for potential applications as surgical implants has been tested on osteoblast- and macrophage-like cells and compared to particles of the Ti6Al4V alloy, for which there is a good clinical experience. After microstructural characterisation of the particles, cells were cultured with particles for 24-48 h using three different concentrations of particles, and the response of cells was quantified by assessment of viability, proliferation, and morphology. Mineralisation by osteoblasts was verified after 21 days. The amount of aluminium and chromium ions in the culture medium of macrophages was measured by graphite furnace atomic absorption and phagocytosis of particles assessed by light microscopy. Viability and proliferation of osteoblast- and macrophage-like cells were substantially unaffected by the presence of particles of the new alloys, which were phagocytosed according to their size. Aluminium and chromium ions were released in the culture medium, but no direct correlation with the cell behaviour was found. In vitro mineralisation was achieved by osteoblasts in due time. The new alloys are well tolerated in in vitro systems, and,...Continue Reading

References

Mar 1, 1982·Journal of Biomedical Materials Research·G C Clark, D F Williams
Nov 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A M MaurerJ S Kawalec
Jan 1, 1995·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·G J Thompson, D A Puleo
Nov 22, 1997·Journal of Biomedical Materials Research·J S SunF H Lin
Aug 6, 1998·Biomaterials·S MoraisC A van Blitterswijk
Dec 18, 2001·Journal of Biomedical Materials Research·Akiko YamamotoTakao Hanawa
Jul 13, 2002·Journal of Biomedical Materials Research·Dominique P PiolettiJacques Corbeil
Aug 31, 2002·Biomolecular Engineering·C J KirkpatrickK Peters
Sep 25, 2004·Journal of Materials Science. Materials in Medicine·M S FloresG Rivero

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Citations

Apr 20, 2007·Journal of Materials Science. Materials in Medicine·Paul RoachCarole C Perry
Feb 15, 2016·Journal of the Mechanical Behavior of Biomedical Materials·E FrutosT Polcar
Feb 6, 2007·Biomaterials·Frank WitteNorbert Hort
Jul 20, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·J L González-CarrascoN Baldini
Jun 12, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Ling RenKe Yang
Nov 17, 2005·The International Journal of Artificial Organs·M TschonR Giardino

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