Quantitative assessments of the cytotoxicity of bladder carcinogens towards cultured normal human uroepithelial cells

Carcinogenesis
C A ReznikoffS Swaminathan

Abstract

Chemical carcinogens are known to exert cytotoxic effects on cells. The survival of cultured human uroepithelial cells (HUC) after exposure to several important classes of human and experimental animal bladder carcinogens has been quantitatively assessed in vitro using reduction in cell number and/or colony forming efficiency as the endpoint(s). Cells were treated with different carcinogens or various metabolites of a procarcinogen and the responses were analyzed with respect to the cell type used and to the donor source of the cells. The cytotoxic responses of HUC to the stable bladder procarcinogens tested [4-aminobiphenyl (ABP), 4-nitrobiphenyl, N-[4-(5-nitro-2-furyl)-2-thiazole]formamide and 2-amino-4-(5-nitro-2-furyl)thiazole] were dependent on both the concentration of chemical used and the duration of exposure. The survival of HUC after exposure to several metabolites of ABP differed. The N-hydroxylated derivatives of ABP (N-hydroxy-4-amino-biphenyl and N-hydroxy-4-acetylaminobiphenyl) were considerably more cytotoxic toward HUC than ABP or 4-acetylaminobiphenyl. The survival of HUC from different individuals after treatment with the direct acting carcinogen N-nitro-N-methylurea was very similar. In contrast, the surviva...Continue Reading

Citations

Sep 1, 1992·Seminars in Surgical Oncology·J A SchalkenF M Debruyne
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·W FöllmannC Guhe
Nov 17, 2005·Carcinogenesis·R A CrallanJ Southgate
Mar 21, 1998·Journal of Occupational and Environmental Medicine·R E SavageD Werren

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