Quantitative autoradiographic analysis of ionotropic glutamate receptor subtypes in human temporal lobe epilepsy: up-regulation in reorganized epileptogenic hippocampus

The European Journal of Neuroscience
M BrinesNihal C de Lanerolle

Abstract

Medically intractable temporal lobe epilepsy is a common disease typically associated with hippocampal damage (sclerosis) and synaptic remodelling. These changes could include increased glutamate receptor expression, enhancing excitability and the potential for neuronal injury. We directly assessed this hypothesis using quantitative in vitro receptor autoradiography to determine the densities of glutamate-, NMDA-, quisqualate/alpha-amino-3-hydroxy-5-methyl-isoxazoleproprionic acid (AMPA)- and kainic acid-preferring binding sites in surgically removed hippocampi from patients with mesial temporal lobe epilepsy (sclerosis; MTLE) and patients with mass-associated temporal lobe epilepsy (no sclerosis; MaTLE), compared with autopsy material. Neuronal cell counts and in situ total protein densities were also obtained. In general, MaTLE and autopsy binding densities were indistinguishable. In contrast, some regions of MTLE hippocampi exhibited decreased receptor densities, with a corresponding loss of protein. In the hilus and CA1, however, ligand binding densities did not differ from the comparison groups in spite of markedly reduced protein content, consistent with increased glutamate receptor density. Kainate-preferring sites were ...Continue Reading

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Citations

Apr 13, 2000·The European Journal of Neuroscience·A SíkT F Freund
Jul 18, 2002·Epilepsia·Gerald SeifertChristian Steinhäuser
Feb 4, 2006·Acta Neuropathologica·Tih-Shih LeeNihal C de Lanerolle
Dec 14, 2004·American Journal of Human Genetics·Florence MolinariLaurence Colleaux
Apr 3, 2007·Pharmacology & Therapeutics·Márcia Renata MortariWagner Ferreira dos Santos
May 29, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Z PfundG J Moore
May 25, 2018·The International Journal of Neuroscience·E Samokhina, Alexander Samokhin
Aug 8, 2006·Medicinal Research Reviews·Daniela CatarziFlavia Varano
Jul 10, 2019·European Journal of Human Genetics : EJHG·Camille LemattreDavid Geneviève
Dec 7, 2010·CNS Neuroscience & Therapeutics·Carlos Matute
Aug 11, 2020·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Angelo LabateAntonio Gambardella

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