Quantitative bioassays for measuring biologically functional gonadotropins based on eel gonadotropic receptors

General and Comparative Endocrinology
Yuki MinegishiG E E J M van den Thillart

Abstract

Significant declines in eel stocks have been noted in many parts of the world. Because eel aquaculture is dependent on wild-caught juveniles, there is a need to achieve artificial reproduction. Adult eel maturation is currently induced by repeated injections of purified gonadotropin (human chorionic gonadotropin [hCG]) or pituitary extract. Thus the determination of the biological efficacy and quantification of internal levels of gonadotropic hormones is important for optimizing artificial reproduction protocols. To quantify the plasma levels of biologically functional gonadotropic hormones, we developed a bioassay for luteinizing hormone (LH) and follicle-stimulating hormone (FSH) based on the stable expression of receptors in HEK293 cells of the Japanese eel Anguilla japonica LH (ajLHR) and the European eel Anguilla anguilla FSH (aaFSHR), respectively. Such cells also contain a firefly luciferase reporter gene driven by a cAMP-responsive element (CRE-Luc). We found that the obtained stable cells, with ajLHR, responded linearly to a more than 100,000-fold concentration range of hCG diluted in saline. The cells with aaFSHR showed a linear response to a 1000-fold concentration range of salmon pituitary extract mixed with saline....Continue Reading

References

Sep 1, 1988·General and Comparative Endocrinology·K SuzukiH Kawauchi
Aug 14, 2003·Journal of Molecular Endocrinology·H F VischerJ Bogerd
May 3, 2005·General and Comparative Endocrinology·Jan BogerdHenry F Vischer
Jun 29, 2007·General and Comparative Endocrinology·Shan-Ru JengChing-Fong Chang
Aug 19, 2009·General and Comparative Endocrinology·B Levavi-SivanJ J Lareyre
Aug 25, 2009·General and Comparative Endocrinology·David S PeñarandaJuan F Asturiano
Sep 22, 2009·General and Comparative Endocrinology·Arjan P PalstraGuido E E J M van den Thillart

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Citations

May 24, 2015·General and Comparative Endocrinology·María José MazónAna Gómez
Aug 22, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Erik BurgerhoutGuido E E J M van den Thillart

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