Quantitative characterization of binding of small molecules to extracellular matrix

Journal of Biochemical and Biophysical Methods
Yufen ZhangStefan Balaz

Abstract

Extracellular matrix (ECM) is a major tissue component that, besides its cell support function, is implicated in cell-cell signaling, wound repair, cell adhesion, and other cell and tissue functions. For small molecules acting in tissues, including chemicals, signaling peptides, effectors, inhibitors, and other man-made and physiological compounds, non-specific binding to ECM is a critical phenomenon affecting their disposition. We describe here a method for a quantitative characterization of the ECM binding, using a solidified ECM layer incubated with medium containing studied small molecules. Working conditions of Matrigel, a commercial basement membrane preparation, were optimized in terms of the protein concentration, surface area, gel layer thickness, solidification time, and mixing speed. The release of proteins from the solidified layer into the buffer was monitored and taken into account. Two major proteins, laminin and collagen IV, dissolve at different rates. The Matrigel stability data, obtained under varying incubation conditions and gentle mixing, can also be useful in other ECM-related research. The experimental binding data, averaged over all binding sites, were analyzed assuming a fast linear binding. The bindin...Continue Reading

References

Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·M Paulsson
Jan 28, 1986·Biochemistry·H K KleinmanG R Martin
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V P TerranovaV Glushko
Jan 1, 1985·Journal of Cellular Biochemistry·V P TorchilinG R Martin
Apr 1, 1981·Biochemical Pharmacology·K J FehskeU Wollert
Apr 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·R E BurgesonJ Sanes
Oct 10, 1998·The Journal of Membrane Biology·H FischerA Seelig
Mar 18, 1999·Journal of Microencapsulation·R CortesiC Nastruzzi
Apr 21, 1999·Current Biology : CB·J Schwarzbauer
Aug 31, 2002·Biophysical Journal·Karin JohnAndreas Herrmann
Oct 24, 2002·Biochemical Pharmacology·Nicole A KratochwilPaul R Gerber
Jul 1, 1959·Virology·R DULBECCO, G FREEMAN
Jun 19, 2012·Drug Discovery Today·Stefan Balaz

❮ Previous
Next ❯

Citations

Jun 6, 2009·Pharmaceutical Research·Qian ZhangMichael S Roberts
Oct 11, 2008·Chemical Biology & Drug Design·Yufen ZhangStefan Balaz
Sep 2, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Justin C JagodinskyMatthew D Hall
Jun 20, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Rabih S TalhoukNajat A Saliba
Mar 2, 2012·Food & Function·Akira Murakami, Kohta Ohnishi
Oct 30, 2019·Journal of Mass Spectrometry : JMS·Jillian JohnsonLingjun Li
Jun 10, 2020·Nature Biomedical Engineering·Nathalie BrandenbergMatthias P Lutolf
Dec 22, 2020·Frontiers in Oncology·Barbora PavlatovskáJarmila Navrátilová
Mar 7, 2009·Chemical Reviews·Stefan Balaz
Nov 18, 2020·Analytical Chemistry·Maura C BelangerRebecca R Pompano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.