Quantitative characterization of T-cell repertoire alteration in Chinese patients with B-cell acute lymphocyte leukemia after CAR-T therapy

Bone Marrow Transplantation
Xiujian WangHe Huang

Abstract

Chimeric antigen receptor (CAR) T-cell therapy has displayed potent anti-leukemia activity in acute lymphocytic leukemia (ALL), acting as a new ray of hope to refractory/relapsed patients. However, the influence of CAR-T therapy on host immune system has not been well elucidated. Thus, We applied high-throughput T cell receptor β chain sequencing to track the dynamic change of T-cell repertoire induced by CAR-T therapy in B-cell ALL patients. Six Chinese patients achieving complete remission were under observation, whose blood samples, bone marrow samples and infused CAR-T samples were collected at serial time points before and after CAR-T therapy. We observed decreased TCR diversity and increased clonality of T-cell repertoire in both peripheral blood and bone marrow after CAR-T administration. The persistent T cell clones in blood and bone marrow expanded following leukemic cell destruction and were barely detected in CAR T-cell pool. For the first time, our results demonstrated CAR-T therapy could stimulate the clonal proliferation of CAR-negative T cells in patients. Considering other groups' animal results indicating that CAR-T therapy could facilitate the proliferation of tumor antigen-specific T cells and that the emerge...Continue Reading

References

Nov 5, 1999·Science·T P ArstilaP Kourilsky
Jun 8, 2000·Science·T P ArstilaP Kourilsky
Mar 26, 2004·Nature Reviews. Immunology·Janko Nikolich-ZugichIlhem Messaoudi
Jul 25, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amorette Barber, Charles L Sentman
Nov 17, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amorette BarberCharles L Sentman
Jan 21, 2012·Nature Immunology·Gerald P Morris, Paul M Allen
Oct 10, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mei GuoHui-Sheng Ai
Oct 15, 2013·Nature Medicine·Carsten LinnemannTon N M Schumacher
Dec 18, 2013·Immunological Reviews·Carsten LinnemannTon N M Schumacher
Feb 21, 2014·Science Translational Medicine·Marco L DavilaRenier Brentjens
Mar 4, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lidia RobertAntoni Ribas
May 30, 2014·Science Translational Medicine·Edward ChaLawrence Fong
Oct 16, 2014·The New England Journal of Medicine·Shannon L MaudeStephan A Grupp
Apr 4, 2015·Science·Steven A Rosenberg, Nicholas P Restifo
Sep 26, 2015·Molecular Oncology·Ilan KirschHarlan Robins
Mar 13, 2016·Cancer Immunology Research·Melody HsuRobert M Prins
Jan 1, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yongxian HuHe Huang
Mar 31, 2017·Journal of Hematology & Oncology·Shengnan YuKongming Wu
May 26, 2017·Nature·Michel SadelainStanley Riddell
May 23, 2018·Journal of Human Genetics·Makda ZewdeYusuke Nakamura

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

GraphPad
GraphPad Prism
R

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Related Papers

JAMA Oncology
John M Pagel, Howard Jack West
Journal of the National Comprehensive Cancer Network : JNCCN
Mauro P Avanzi, Renier J Brentjens
© 2021 Meta ULC. All rights reserved